About: Efficacy loss of strobilurins used in protection against apple scab in Czech orchards

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The apple scab caused by Venturia inaequalis is important apple disease in temperate zone. Economical losses caused by the pathogen are effectively reduced by intensive chemical protection and breeding. Occurrences of pathogen resistance to strobilurin fungicides were noted in some Czech orchards in the past. In 2007-2011, collection of 136 isolates V inaequalis was tested with the aim to detect presence of single-point mutations of cytochrom b (cytb) gene causing efficacy loss of strobilurins. Consequently occurrence of beta-tubulin (beta-tub) gene mutation causing resistance to benomyl was studied with the aim to obtain comparative data about frequency of mutation in non-selective environment. Therefore simple and highly repeatable PCR based mutation detecting methods were optimised. Analysis brought knowledge about high similarity of cytb and beta-tub genes mutations occurrence. Whilst the frequency of the G143A substitution in cytb gene was 65% the E198A substitution of beta-tub gene occurred in 54% within pathogen population. This high frequency of resistant population necessitates revision of the way of strobilurins use in apple scab management. The apple scab caused by Venturia inaequalis is important apple disease in temperate zone. Economical losses caused by the pathogen are effectively reduced by intensive chemical protection and breeding. Occurrences of pathogen resistance to strobilurin fungicides were noted in some Czech orchards in the past. In 2007-2011, collection of 136 isolates V inaequalis was tested with the aim to detect presence of single-point mutations of cytochrom b (cytb) gene causing efficacy loss of strobilurins. Consequently occurrence of beta-tubulin (beta-tub) gene mutation causing resistance to benomyl was studied with the aim to obtain comparative data about frequency of mutation in non-selective environment. Therefore simple and highly repeatable PCR based mutation detecting methods were optimised. Analysis brought knowledge about high similarity of cytb and beta-tub genes mutations occurrence. Whilst the frequency of the G143A substitution in cytb gene was 65% the E198A substitution of beta-tub gene occurred in 54% within pathogen population. This high frequency of resistant population necessitates revision of the way of strobilurins use in apple scab management. (en)
Title	Efficacy loss of strobilurins used in protection against apple scab in Czech orchards Efficacy loss of strobilurins used in protection against apple scab in Czech orchards (en)
skos:prefLabel	Efficacy loss of strobilurins used in protection against apple scab in Czech orchards Efficacy loss of strobilurins used in protection against apple scab in Czech orchards (en)
skos:notation	RIV/62156489:43510/13:00213542!RIV14-MZE-43510___
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(QH71172)
http://linked.open...iv/cisloPeriodika	2
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Boček, Stanislav
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Mendelova univerzita v Brně / Zahradnická fakulta (Lednice)
http://linked.open...dnocenehoVysledku	72021
http://linked.open...ai/riv/idVysledku	RIV/62156489:43510/13:00213542
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	cytochrom b gene; Venturia inaequalis; resistance to fungicides; PCR based detection of mutations (en)
http://linked.open.../riv/klicoveSlovo	PCR based detection of mutations cytochrom b gene resistance to fungicides Venturia inaequalis
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[16545EE6D80F]
http://linked.open...i/riv/nazevZdroje	Horticultural Science
http://linked.open...in/vavai/riv/obor	GF
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Identification of apple scab strains and races (Venturia inaequalis Cke. Wint)
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	40
http://linked.open...iv/tvurceVysledku	Boček, Stanislav Kloutvorová, Jana Sedlák, Petr Vejl, Pavel Vávra, Radek
http://linked.open...ain/vavai/riv/wos	319301900001
issn	0862-867X
number of pages	7 (xsd:int)
http://localhost/t...ganizacniJednotka	43510

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