About: Genetic analysis of the genus Diospyros ssp. using RAPD and i-PBS methods     Goto   Sponge   NotDistinct   Permalink

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  • Molecular techniques RAPD and i-PBS were used to study genetic diversity within persimmon collection at Horticulture Faculty of Mendel University in Lednice. The aim of the work was to distinguish 14 known and 6 of unknown origin persimmon cultivars. The basic screening of 20 OPT primers was applied to 4 cultivars differing in the place of origin. Selected primers were used for the RAPD reactions within the whole persimmon collection. Three OPA primers previously described in the literature were also used for the RAPD reactions within the whole persimmon collection. Additional 16 i-PBS primers previously described in the literature were also used for i-PBS analysis of the whole group of cultivars. Amplification was successful with 12 i-PBS primers. The FreeTree software package was used to generate a similarity matrix and then to produce a dendrogram using UPGMA analyses. The similarity dendrograms of all persimmon cultivars were created based on both approaches and also on combination of both analyses by program Tree View. All the dendrograms clearly separated the assessed cultivars into 4 clusters.
  • Molecular techniques RAPD and i-PBS were used to study genetic diversity within persimmon collection at Horticulture Faculty of Mendel University in Lednice. The aim of the work was to distinguish 14 known and 6 of unknown origin persimmon cultivars. The basic screening of 20 OPT primers was applied to 4 cultivars differing in the place of origin. Selected primers were used for the RAPD reactions within the whole persimmon collection. Three OPA primers previously described in the literature were also used for the RAPD reactions within the whole persimmon collection. Additional 16 i-PBS primers previously described in the literature were also used for i-PBS analysis of the whole group of cultivars. Amplification was successful with 12 i-PBS primers. The FreeTree software package was used to generate a similarity matrix and then to produce a dendrogram using UPGMA analyses. The similarity dendrograms of all persimmon cultivars were created based on both approaches and also on combination of both analyses by program Tree View. All the dendrograms clearly separated the assessed cultivars into 4 clusters. (en)
Title
  • Genetic analysis of the genus Diospyros ssp. using RAPD and i-PBS methods
  • Genetic analysis of the genus Diospyros ssp. using RAPD and i-PBS methods (en)
skos:prefLabel
  • Genetic analysis of the genus Diospyros ssp. using RAPD and i-PBS methods
  • Genetic analysis of the genus Diospyros ssp. using RAPD and i-PBS methods (en)
skos:notation
  • RIV/62156489:43510/12:00193564!RIV13-MSM-43510___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • S
http://linked.open...iv/cisloPeriodika
  • 8
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 137889
http://linked.open...ai/riv/idVysledku
  • RIV/62156489:43510/12:00193564
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • RAPD; dendrogram; persimmon; i-PBS (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • CZ - Česká republika
http://linked.open...ontrolniKodProRIV
  • [369197BC6FFA]
http://linked.open...i/riv/nazevZdroje
  • Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 60
http://linked.open...iv/tvurceVysledku
  • Ondrášek, Ivo
  • Raddová, Jana
  • Čechová, Jana
  • Ptáčková, Hana
issn
  • 1211-8516
number of pages
http://localhost/t...ganizacniJednotka
  • 43510
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