About: Genetic variability of Grapevine fanleaf virus isolates within genes 1B(HEL) and 1E(POL)

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Genetic variability of Grapevine fanleaf virus isolates within genes 1B(HEL) and 1E(POL) Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The genetic variability of six isolates of Grapevine fanleaf virus (GFLV) from South Moravia (Czech Republic) and Italy was investigated by RT-PCR and sequencing within part of the RNA-1-encoded genes 1B(Hel) (379 nts) and 1E(Pol) (365 nts). Sequence analyses showed 96-100% and 91.4-100% identity at the amino acid level for genes 1B(Hel) and 1E(Pol), respectively, among Czech, Italian and other isolates for which sequence information is available. As expected, some of the conserved motifs of the helicase and polymerase proteins were found in the deduced amino acid sequences, although isolates PN33 from Czech Republic, and isolates 55TK and UR11 from Italy had a Ile to Val subtitution in the helicase motif B. Phylogenetic analyses confirmed the close relationship among GFLV isolates and the relatedness of GFLV isolate WAPN173 with Arabis mosaic virus (ArMV) in protein 1B(Hel). Two distinct phylogenetic clusters were identified for protein 1E(Pol), with isolates from California and the French reference strain F13 grouping together, and isolates from the Czech Republic, Italy, New Zealand, and USA grouping in a different lineage. This study provides new insights into the genetic variability of GFLV RNA-1-encoded genes 1B(Hel) and 1E(Pol). The genetic variability of six isolates of Grapevine fanleaf virus (GFLV) from South Moravia (Czech Republic) and Italy was investigated by RT-PCR and sequencing within part of the RNA-1-encoded genes 1B(Hel) (379 nts) and 1E(Pol) (365 nts). Sequence analyses showed 96-100% and 91.4-100% identity at the amino acid level for genes 1B(Hel) and 1E(Pol), respectively, among Czech, Italian and other isolates for which sequence information is available. As expected, some of the conserved motifs of the helicase and polymerase proteins were found in the deduced amino acid sequences, although isolates PN33 from Czech Republic, and isolates 55TK and UR11 from Italy had a Ile to Val subtitution in the helicase motif B. Phylogenetic analyses confirmed the close relationship among GFLV isolates and the relatedness of GFLV isolate WAPN173 with Arabis mosaic virus (ArMV) in protein 1B(Hel). Two distinct phylogenetic clusters were identified for protein 1E(Pol), with isolates from California and the French reference strain F13 grouping together, and isolates from the Czech Republic, Italy, New Zealand, and USA grouping in a different lineage. This study provides new insights into the genetic variability of GFLV RNA-1-encoded genes 1B(Hel) and 1E(Pol). (en)
Title	Genetic variability of Grapevine fanleaf virus isolates within genes 1B(HEL) and 1E(POL) Genetic variability of Grapevine fanleaf virus isolates within genes 1B(HEL) and 1E(POL) (en)
skos:prefLabel	Genetic variability of Grapevine fanleaf virus isolates within genes 1B(HEL) and 1E(POL) Genetic variability of Grapevine fanleaf virus isolates within genes 1B(HEL) and 1E(POL) (en)
skos:notation	RIV/62156489:43510/11:00174486!RIV12-MZE-43510___
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(QH91214)
http://linked.open...iv/cisloPeriodika	2
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Pidra, Miroslav Eichmeier, Aleš Baránek, Miroslav
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Mendelova univerzita v Brně / Zahradnická fakulta (Lednice)
http://linked.open...dnocenehoVysledku	201012
http://linked.open...ai/riv/idVysledku	RIV/62156489:43510/11:00174486
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	phylogeny; polymerase; Grapevine fanleaf virus; molecular variability; helicase (en)
http://linked.open.../riv/klicoveSlovo	Grapevine fanleaf virus helicase molecular variability polymerase phylogeny
http://linked.open...odStatuVydavatele	IT - Italská republika
http://linked.open...ontrolniKodProRIV	[3D5C53A3D8E5]
http://linked.open...i/riv/nazevZdroje	Journal of Plant Pathology
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	New biotechnolgical approaches for nepovirus resitance creation in grapevine rootstock cultivars.
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	93
http://linked.open...iv/tvurceVysledku	Pidra, Miroslav Baránek, Miroslav Eichmeier, Aleš
http://linked.open...ain/vavai/riv/wos	293602400034
issn	1125-4653
number of pages	5 (xsd:int)
http://localhost/t...ganizacniJednotka	43510

Faceted Search & Find service v1.16.118 as of Jun 21 2024

Alternative Linked Data Documents: ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3240 as of Jun 21 2024, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (126 GB total memory, 48 GB memory in use)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software