About: Optimization of the Preparation of ds cDNA from Flower Buds of Apricot for the cDNA-AFLP Analysis     Goto   Sponge   NotDistinct   Permalink

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  • The aim of the work was to develop the preparation of ds cDNA from flower buds of four apricot varieties ('Sundrop', 'Stark Early Orange', 'Vestar' and 'Betinka') so that it would be as suitable as possible for the cDNA-AFLP analysis. The sampled tissues were preserved using a commercial agent RNAlater Soln. (Ambion). The RNA from the chosen samples was isolated using a commercial isolation kit supplied by Sigma (SpectrumTM Plant Total RNA Kit), which support the isolation of sufficient amounts of high quality RNA from these highly complicated tissues. Transcription from the entire RNA onto the ds cDNA was achieved using the MINT cDNA synthesis kit from Evrogen. The cDNA-AFLP analysis was carried out according to an amended procedure by BACHEM et al. (1998) and the samples obtained from selective amplification were analysed on genetic analyser ABI-PRISM 310. The results obtained by another protocol (include silica extraction of RNA, GenElute mRNA Miniprep Kit and others) were compared with new ones. O
  • The aim of the work was to develop the preparation of ds cDNA from flower buds of four apricot varieties ('Sundrop', 'Stark Early Orange', 'Vestar' and 'Betinka') so that it would be as suitable as possible for the cDNA-AFLP analysis. The sampled tissues were preserved using a commercial agent RNAlater Soln. (Ambion). The RNA from the chosen samples was isolated using a commercial isolation kit supplied by Sigma (SpectrumTM Plant Total RNA Kit), which support the isolation of sufficient amounts of high quality RNA from these highly complicated tissues. Transcription from the entire RNA onto the ds cDNA was achieved using the MINT cDNA synthesis kit from Evrogen. The cDNA-AFLP analysis was carried out according to an amended procedure by BACHEM et al. (1998) and the samples obtained from selective amplification were analysed on genetic analyser ABI-PRISM 310. The results obtained by another protocol (include silica extraction of RNA, GenElute mRNA Miniprep Kit and others) were compared with new ones. O (en)
Title
  • Optimization of the Preparation of ds cDNA from Flower Buds of Apricot for the cDNA-AFLP Analysis
  • Optimization of the Preparation of ds cDNA from Flower Buds of Apricot for the cDNA-AFLP Analysis (en)
skos:prefLabel
  • Optimization of the Preparation of ds cDNA from Flower Buds of Apricot for the cDNA-AFLP Analysis
  • Optimization of the Preparation of ds cDNA from Flower Buds of Apricot for the cDNA-AFLP Analysis (en)
skos:notation
  • RIV/62156489:43510/09:00170204!RIV11-MSM-43510___
http://linked.open...avai/riv/aktivita
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  • S
http://linked.open...iv/cisloPeriodika
  • 839
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http://linked.open...aciTvurceVysledku
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  • 332028
http://linked.open...ai/riv/idVysledku
  • RIV/62156489:43510/09:00170204
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  • Spectrum Plant Total RNA Kit; MINT cDNA Synthesis Kit; RNA later (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • BE - Belgické království
http://linked.open...ontrolniKodProRIV
  • [534B35C56926]
http://linked.open...i/riv/nazevZdroje
  • Acta horticulturae
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...UplatneniVysledku
http://linked.open...iv/tvurceVysledku
  • Pidra, Miroslav
  • Baránek, Miroslav
  • Čechová, Jana
issn
  • 0567-7572
number of pages
http://localhost/t...ganizacniJednotka
  • 43510
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