About: Suitability of the date of grapevine tissue sampling for tests of gflv and armv presence using elisa method     Goto   Sponge   NotDistinct   Permalink

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Description
  • Významnými virovými chorobami ze skupiny nepovirů jsou roncet révy vinné -- GFLV (grapevine fanleaf virus) a mozaika hůseníku u révy vinné -- ArMV (arabis mosaic virus on grapevine). Serodiagnostika je v současné době jednou z rutinních metod testování virových chorob, i u révy vinné. Cílem práce je nalezení vhodného termínu odběru vzorku z révové rostliny na základě otestování ELISA metodou a pozorování vizuálních příznaků GFVL a ArMV. Pozitivně hodnocené na přítomnost GFLV byly vzorky v následujících termínech B (intenzivní růst -- vegetační vrchol), C (začátek kvetení -- mladý list) a D (začátek kvetení -- květenství). Pozitivně hodnocené vzorky na přítomnost ArMV metodou ELISA byly zjištěny v termínech B ( intenzivní růst -- vegetační vrchol) a C (začátek kvetení -- květenství). Hodnocení ve zbývajících termínech bylo negativní. V našem víceletém sledování se potvrdily výsledky, které uvádí BOUYAHIA et al. (2003) a WEBER (2002). Jako nejvhodnější termín odběru a část rostliny, se ukazuje term (cs)
  • Grapevine fanleaf virus (GFLV) and Arabis mosaic virus on grapevine (ArMV) are two important agents from the group of nepoviruses. At present, serodiagnostic method is one of routine methods of virus diseases testing also in grapevines. The aim of this study was to determine a suitable date of grapevine tissue sampling using the ELISA method and to follow visible symptoms of GFVL and ArMV infestation of vines. Positive results of tests for the occurrence of GFLV were obtained in the following growth stages: B (intensive growth -- growth apex), C (beginning of flowering -- young leaves) and D (beginning of flowering -- inflorescence). Samples evaluated by ELISA method as positive for ArMV were found out in growth stages B (intensive growth -- growth apex) and C (beginning of flowering -- inflorescence). In all other stages the results of performed tests were negative. It is concluded that the stage B (intensive growth -- growth apex) seems to be the most suitable for sampling of grapevine tissue.
  • Grapevine fanleaf virus (GFLV) and Arabis mosaic virus on grapevine (ArMV) are two important agents from the group of nepoviruses. At present, serodiagnostic method is one of routine methods of virus diseases testing also in grapevines. The aim of this study was to determine a suitable date of grapevine tissue sampling using the ELISA method and to follow visible symptoms of GFVL and ArMV infestation of vines. Positive results of tests for the occurrence of GFLV were obtained in the following growth stages: B (intensive growth -- growth apex), C (beginning of flowering -- young leaves) and D (beginning of flowering -- inflorescence). Samples evaluated by ELISA method as positive for ArMV were found out in growth stages B (intensive growth -- growth apex) and C (beginning of flowering -- inflorescence). In all other stages the results of performed tests were negative. It is concluded that the stage B (intensive growth -- growth apex) seems to be the most suitable for sampling of grapevine tissue. (en)
Title
  • Suitability of the date of grapevine tissue sampling for tests of gflv and armv presence using elisa method
  • Vhodnost termínu odběru vzorku révy vinné pro testování GFLV a ArMV metodou ELISA (cs)
  • Suitability of the date of grapevine tissue sampling for tests of gflv and armv presence using elisa method (en)
skos:prefLabel
  • Suitability of the date of grapevine tissue sampling for tests of gflv and armv presence using elisa method
  • Vhodnost termínu odběru vzorku révy vinné pro testování GFLV a ArMV metodou ELISA (cs)
  • Suitability of the date of grapevine tissue sampling for tests of gflv and armv presence using elisa method (en)
skos:notation
  • RIV/62156489:43510/07:00115966!RIV08-MZE-43510___
http://linked.open.../vavai/riv/strany
  • 223;228
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(QC0048)
http://linked.open...iv/cisloPeriodika
  • 5
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 453485
http://linked.open...ai/riv/idVysledku
  • RIV/62156489:43510/07:00115966
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • grapevine; grapevine virus disease; GFLV; ArMV; ELISA method; visual symptoms; term of sampling (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • CZ - Česká republika
http://linked.open...ontrolniKodProRIV
  • [B87D9439833B]
http://linked.open...i/riv/nazevZdroje
  • Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • LV
http://linked.open...iv/tvurceVysledku
  • Pavloušek, Pavel
issn
  • 1211-8516
number of pages
http://localhost/t...ganizacniJednotka
  • 43510
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