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| rdf:type
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| Description
| - For the selection of resistent genotypes of breeding material in Callistephus chinensis NEES. against Fusarium strains, it was searched for specific active strains of this. Gene pool of Fusarium strains was collected, purified and tested with plant material. To declare differences between pathogenic and non-pathogenic isolates were genomic methods used. The RAPD and PCR technique (Moravcová et al. 2004, Pasquali et al. 2003) were used to analyse of genomic DNA of 40 fusarium strains, collected from different host plants, mainly Callistephus chinensis NEES. in comparison with 13 Fusarium species included F. oxysporum and its f.sp. callistephi, cyclaminis, dianthi, lycopersici, radicis-lycopersici, narcisi, pisi and tracheiphilum. These isolates are deposited in University fungal collection. 10 deca-hexamers (Pasquali et al. 2003, Hennig 1999) random primers and 2 pairs of specific primers were used for amplification in these study. New PCR markers based on conservative sequences in mitochondrial Fusar
- For the selection of resistent genotypes of breeding material in Callistephus chinensis NEES. against Fusarium strains, it was searched for specific active strains of this. Gene pool of Fusarium strains was collected, purified and tested with plant material. To declare differences between pathogenic and non-pathogenic isolates were genomic methods used. The RAPD and PCR technique (Moravcová et al. 2004, Pasquali et al. 2003) were used to analyse of genomic DNA of 40 fusarium strains, collected from different host plants, mainly Callistephus chinensis NEES. in comparison with 13 Fusarium species included F. oxysporum and its f.sp. callistephi, cyclaminis, dianthi, lycopersici, radicis-lycopersici, narcisi, pisi and tracheiphilum. These isolates are deposited in University fungal collection. 10 deca-hexamers (Pasquali et al. 2003, Hennig 1999) random primers and 2 pairs of specific primers were used for amplification in these study. New PCR markers based on conservative sequences in mitochondrial Fusar (en)
- Při selekci citlivosti rezistentních genotypů ve šlechtitelském materiálu Callistephus chinensis NEES. vůči Fusarium byly hledány jeho specifické aktivní formy. Byl sousředěn genový matereiál rodu Fusarium, izolován, purifikován a testován na rostlinách. Pro objasnění rozdílů patogenních a nepatogenních isolátů bylo užito genomických metod. RAPD a PCR techniky (Moravcová et al. 2004, Pasquali et al. 2003) byly použity k analýze DNA u 40 izolátů fusarií, získaných z různých hostitelských rostlin, především od C. chinensis NEES. Ve srovnání s 13 spec. formami včetně F. oxysporum a jeho f. speciales callistephi, cyclaminis, dianthi, lycoperssici radicis-lycopersici, narcisi, pisi and tracheiphylum. Tyto isoláty jsou uloženy v universitní kolekci houbových patogenů. K amplifikacibyly využity v této studii 10 deca-hexamers (Pasquali et al.2003, Hennig 1999) náhodných primeru a 2 páry specifických primerů.Byly testovány nové PCR markery založené na bázi mitochondriálních ..... (cs)
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| Title
| - Characterization of Fusarium spp. and formae speciales using RAPD and PCR markers
- Characterization of Fusarium spp. and formae speciales using RAPD and PCR markers (en)
- Stanovení Fusarium spp. a forma specialis pomocí RAPD a PCR markerů. (cs)
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| skos:prefLabel
| - Characterization of Fusarium spp. and formae speciales using RAPD and PCR markers
- Characterization of Fusarium spp. and formae speciales using RAPD and PCR markers (en)
- Stanovení Fusarium spp. a forma specialis pomocí RAPD a PCR markerů. (cs)
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| skos:notation
| - RIV/62156489:43510/06:00103982!RIV07-MSM-43510___
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/62156489:43510/06:00103982
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - Fusarium, Callistephus, test of resistence; RAPD, PCR techniques (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...UplatneniVysledku
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| http://linked.open...iv/tvurceVysledku
| - Baránek, Miroslav
- Kobza, František
- Kulhánek, Tomáš
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| http://linked.open...n/vavai/riv/zamer
| |
| http://localhost/t...ganizacniJednotka
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