About: Use of SSR markers to identify grapevine cultivars registered in the Czech republic

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Pou?ití SSR markerů pro identifikaci odrůd révy registrovaných v České republice (cs) In this study we analyzed 51 grapevine cultivars registered in the Czech Republic using six highly informative SSR loci, and determined SSR profiles typical for each cultivar. All cultivars were easily distinguished on the basis of SSR allele length, except for berry color mutants (Pinot and Chasselas group). The average number of detected alleles per locus was 13.2, while observed heterozygosity is between 0.745 (VrZAG 79) and 0.922 (VVMD 7). The lengths of the alleles were compared with results from other laboratories; lengths were either the same or different by 1-3 bp. We also tested a new approach to microsatellite data comparison using a coding system based on reference alleles as a size standard (THIS et al., 2004). The obtained profiles were mostly in accordance with profiles of reference cultivars (THIS et al., 2004); we discuss the possible reasons for observed discrepancies. A third allele in the microsatellite spectrum was observed in five samples where template DNA originated fr In this study we analyzed 51 grapevine cultivars registered in the Czech Republic using six highly informative SSR loci, and determined SSR profiles typical for each cultivar. All cultivars were easily distinguished on the basis of SSR allele length, except for berry color mutants (Pinot and Chasselas group). The average number of detected alleles per locus was 13.2, while observed heterozygosity is between 0.745 (VrZAG 79) and 0.922 (VVMD 7). The lengths of the alleles were compared with results from other laboratories; lengths were either the same or different by 1-3 bp. We also tested a new approach to microsatellite data comparison using a coding system based on reference alleles as a size standard (THIS et al., 2004). The obtained profiles were mostly in accordance with profiles of reference cultivars (THIS et al., 2004); we discuss the possible reasons for observed discrepancies. A third allele in the microsatellite spectrum was observed in five samples where template DNA originated fr (en)
Title	Use of SSR markers to identify grapevine cultivars registered in the Czech republic Pou?ití SSR markerů pro identifikaci odrůd révy registrovaných v České republice (cs) Use of SSR markers to identify grapevine cultivars registered in the Czech republic (en)
skos:prefLabel	Use of SSR markers to identify grapevine cultivars registered in the Czech republic Pou?ití SSR markerů pro identifikaci odrůd révy registrovaných v České republice (cs) Use of SSR markers to identify grapevine cultivars registered in the Czech republic (en)
skos:notation	RIV/62156489:43510/06:00094517!RIV07-MZE-43510___
http://linked.open.../vavai/riv/strany	2
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(QC1156)
http://linked.open...iv/cisloPeriodika	2
http://linked.open...vai/riv/dodaniDat	2007
http://linked.open...aciTvurceVysledku	Baránková, Kateřina Pidra, Miroslav Baránek, Miroslav
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Mendelova univerzita v Brně / Zahradnická fakulta (Lednice)
http://linked.open...dnocenehoVysledku	505322
http://linked.open...ai/riv/idVysledku	RIV/62156489:43510/06:00094517
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	SSR, Vitis vinifera, cultivar identification, genotyping (en)
http://linked.open.../riv/klicoveSlovo	SSR Vitis vinifera cultivar identification genotyping
http://linked.open...odStatuVydavatele	FR - Francouzská republika
http://linked.open...ontrolniKodProRIV	[4C1058E2C7F8]
http://linked.open...i/riv/nazevZdroje	International journal of vine and wine sciences
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Creation of alterntive methodology for identification of Vitis genus cultivars based on evaluation differencies of its DNA structure.
http://linked.open...UplatneniVysledku	2006
http://linked.open...iv/tvurceVysledku	Pidra, Miroslav Baránek, Miroslav Moravcová, Kateřina
issn	1151-0285
number of pages	10 (xsd:int)
http://localhost/t...ganizacniJednotka	43510

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