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Description
| - The aim of this work was to to investigate the suitability of various types of extractants for extraction of acid phosphomonoesterase from soil. Succinate-borate buff er of pH 4.8 or 0.1 M K2SO4 were the most effi cient to extract this enzyme compared to 0.1 M glutamic acid or 0.005 M salicylic acid. Extraction using 0.1 M glutamic acid gave signifi cantly (P<0.05) lowest extraction yield. The following extracts were obtained: clear K2SO4 and glutamic acid extracts, succinate-borate buff er extracts were of slight coloration, and in some cases, rose-colored extracts of salicylic acid. The results of this work are in accordance with low extraction yields of phosphomonoesterase reported in other studies.
- The aim of this work was to to investigate the suitability of various types of extractants for extraction of acid phosphomonoesterase from soil. Succinate-borate buff er of pH 4.8 or 0.1 M K2SO4 were the most effi cient to extract this enzyme compared to 0.1 M glutamic acid or 0.005 M salicylic acid. Extraction using 0.1 M glutamic acid gave signifi cantly (P<0.05) lowest extraction yield. The following extracts were obtained: clear K2SO4 and glutamic acid extracts, succinate-borate buff er extracts were of slight coloration, and in some cases, rose-colored extracts of salicylic acid. The results of this work are in accordance with low extraction yields of phosphomonoesterase reported in other studies. (en)
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Title
| - Extraction of acid phosphomonoesterase from soil: testing of various extractants.
- Extraction of acid phosphomonoesterase from soil: testing of various extractants. (en)
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skos:prefLabel
| - Extraction of acid phosphomonoesterase from soil: testing of various extractants.
- Extraction of acid phosphomonoesterase from soil: testing of various extractants. (en)
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skos:notation
| - RIV/62156489:43410/11:00169235!RIV12-MSM-43410___
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/62156489:43410/11:00169235
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - salicylic acid; půda; glutamic acid; K2SO4; acid phosphomonoesterase (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
| - Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Dundek, Peter
- Formánek, Pavel
- Holík, Ladislav
- Janouš, Dalibor
- Rejšek, Klement
- Vranová, Valerie
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http://linked.open...n/vavai/riv/zamer
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issn
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number of pages
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http://localhost/t...ganizacniJednotka
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