About: Effect of sucrose concentration, polyethylene glycol and activated charcoal on maturation and regeneration of Abies cephalonica somatic embryos     Goto   Sponge   NotDistinct   Permalink

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  • For promoting the maturation of two embryogenic cell lines of Abies cephalonica Loud., the effect of two sucrose concentrations (87.6 and 175.2 mM) applied alone (Control) or in combination with activated charcoal (AC) (1 week) or with polyethylene glycol (PEG1 and PEG4) (1 or 4 weeks) was studied. The effect of each maturation medium was tested with four concentrations of abscisic acid (4, 8, 16 and 32 lM ABA). AC supplement significantly increased the percentage of embryogenic cell masses (ECMs) with cotyledonary embryos but did not affect the average number of embryos per 1 g of ECMs fresh weight. The highest percentages of ECMs (77.5%) with cotyledonary somatic embryos and the highest average number of somatic embryos (36.5 +- 13.4) were found on media with PEG for 4 weeks. The maturation media with 87.6 mM sucrose significantly increased the number of ECMs able to form cotyledonary embryos (66.8%) when PEG was included in the maturation medium, but did not affect the average num
  • For promoting the maturation of two embryogenic cell lines of Abies cephalonica Loud., the effect of two sucrose concentrations (87.6 and 175.2 mM) applied alone (Control) or in combination with activated charcoal (AC) (1 week) or with polyethylene glycol (PEG1 and PEG4) (1 or 4 weeks) was studied. The effect of each maturation medium was tested with four concentrations of abscisic acid (4, 8, 16 and 32 lM ABA). AC supplement significantly increased the percentage of embryogenic cell masses (ECMs) with cotyledonary embryos but did not affect the average number of embryos per 1 g of ECMs fresh weight. The highest percentages of ECMs (77.5%) with cotyledonary somatic embryos and the highest average number of somatic embryos (36.5 +- 13.4) were found on media with PEG for 4 weeks. The maturation media with 87.6 mM sucrose significantly increased the number of ECMs able to form cotyledonary embryos (66.8%) when PEG was included in the maturation medium, but did not affect the average num (en)
Title
  • Effect of sucrose concentration, polyethylene glycol and activated charcoal on maturation and regeneration of Abies cephalonica somatic embryos
  • Effect of sucrose concentration, polyethylene glycol and activated charcoal on maturation and regeneration of Abies cephalonica somatic embryos (en)
skos:prefLabel
  • Effect of sucrose concentration, polyethylene glycol and activated charcoal on maturation and regeneration of Abies cephalonica somatic embryos
  • Effect of sucrose concentration, polyethylene glycol and activated charcoal on maturation and regeneration of Abies cephalonica somatic embryos (en)
skos:notation
  • RIV/62156489:43410/09:00146517!RIV11-MSM-43410___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • S
http://linked.open...iv/cisloPeriodika
  • 3
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 312269
http://linked.open...ai/riv/idVysledku
  • RIV/62156489:43410/09:00146517
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • somatic embryo (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • NL - Nizozemsko
http://linked.open...ontrolniKodProRIV
  • [263F9DC7DC35]
http://linked.open...i/riv/nazevZdroje
  • Plant cell tissue and organ culture
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 96
http://linked.open...iv/tvurceVysledku
  • Krajňáková, Jana
  • GÖMÖRY, DUŠAN
  • HÄGGMAN, HELY
issn
  • 0167-6857
number of pages
http://localhost/t...ganizacniJednotka
  • 43410
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