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rdf:type
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Description
| - The method for supercritical fluid extraction (SFE) of amino acids was adapted and optimized for a dry leaves matrix. The matrix-dependent SFE method uses a mixture of MeOH:H2O:acetonitrile (10:10:1 v/v) as a modifier (0.5 ml in situ) at 70 °C and 40 MPa and no HCl is needed as an entrainer. The amino acids were quantified using high-performance liquid chromatography with fluorimetric detection (HPLC/FLD) after gradient elution on Zorbax Eclipse columns (4.6 x 75 mm) with aqueous Na2HPO4 buffer of pH 7.8 and ACN:MeOH:water as a mobile phase. In comparison with Soxhlet extraction, SFE gained higher recovery and selectivity, but it required longer extraction time (90 min) and it was more labor-intensive (clean-up step after the pre-concentration). Both methods should be used separately or as a combo according to the matrix, number of samples and levels of ballast compounds.
- The method for supercritical fluid extraction (SFE) of amino acids was adapted and optimized for a dry leaves matrix. The matrix-dependent SFE method uses a mixture of MeOH:H2O:acetonitrile (10:10:1 v/v) as a modifier (0.5 ml in situ) at 70 °C and 40 MPa and no HCl is needed as an entrainer. The amino acids were quantified using high-performance liquid chromatography with fluorimetric detection (HPLC/FLD) after gradient elution on Zorbax Eclipse columns (4.6 x 75 mm) with aqueous Na2HPO4 buffer of pH 7.8 and ACN:MeOH:water as a mobile phase. In comparison with Soxhlet extraction, SFE gained higher recovery and selectivity, but it required longer extraction time (90 min) and it was more labor-intensive (clean-up step after the pre-concentration). Both methods should be used separately or as a combo according to the matrix, number of samples and levels of ballast compounds. (en)
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Title
| - Supercritical Fluid Extraction of Amino Acids from Birch (Betula pendula Roth) Leaves and their Liquid Chromatographic Determination with Fluorimetric Detection
- Supercritical Fluid Extraction of Amino Acids from Birch (Betula pendula Roth) Leaves and their Liquid Chromatographic Determination with Fluorimetric Detection (en)
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skos:prefLabel
| - Supercritical Fluid Extraction of Amino Acids from Birch (Betula pendula Roth) Leaves and their Liquid Chromatographic Determination with Fluorimetric Detection
- Supercritical Fluid Extraction of Amino Acids from Birch (Betula pendula Roth) Leaves and their Liquid Chromatographic Determination with Fluorimetric Detection (en)
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skos:notation
| - RIV/62156489:43410/08:00116481!RIV12-GA0-43410___
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
| - P(GA526/05/0187), Z(MSM6215648902)
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/62156489:43410/08:00116481
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - birch leaves; chromatography; amino acids (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
| - DE - Spolková republika Německo
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
| - Journal of Separation Science
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Kula, Emanuel
- Kubáň, Vlastimil
- Klejdus, Bořivoj
- Hrdlička, Petr
- Lojková, Lea
- Buchta, Ivan
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http://linked.open...n/vavai/riv/zamer
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issn
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number of pages
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http://localhost/t...ganizacniJednotka
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