About: Granule-bound starch synthase and endosperm mealiness correlaton in wheat (Triticum aestivum L.) with nonstandard colored caryopses     Goto   Sponge   NotDistinct   Permalink

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Description
  • Granule-bound starch synthase (GBSS) is a key enzyme in starch biosynthesis pathway. It is responsible for production of amylose, therefore low amylose level has been associated with GBSS deficiency. This enzyme, also known as waxy protein, is encoded by three homologous genes -- Wx-A1, Wx-B1 and Wx-D1 and their dysfunction of any origin causes decrease of amylose content. Starch content and, therefore amylose content, is closely linked with structure of the cereal endosperm. It is known, that mealy endosperm contains more starch than vitreous, which contains more proteins. To identify null alleles of waxy genes in 25 genotypes of wheat with nonstandard colored caryopses, PCR markers were used. To determine the endosperm structure of these genotypes, the non-destructive Light Transflectance Meter (LTm) was used. Null alleles for Wx-B1 locus were observed in 5 tested genotypes, loci Wx-A1 and Wx-D1 were amplified in all genotypes. Percentage of mealy caryopses per sample ranged from 0 to 100 % among tested genotypes. No significant correlation between these two parameters was observed.
  • Granule-bound starch synthase (GBSS) is a key enzyme in starch biosynthesis pathway. It is responsible for production of amylose, therefore low amylose level has been associated with GBSS deficiency. This enzyme, also known as waxy protein, is encoded by three homologous genes -- Wx-A1, Wx-B1 and Wx-D1 and their dysfunction of any origin causes decrease of amylose content. Starch content and, therefore amylose content, is closely linked with structure of the cereal endosperm. It is known, that mealy endosperm contains more starch than vitreous, which contains more proteins. To identify null alleles of waxy genes in 25 genotypes of wheat with nonstandard colored caryopses, PCR markers were used. To determine the endosperm structure of these genotypes, the non-destructive Light Transflectance Meter (LTm) was used. Null alleles for Wx-B1 locus were observed in 5 tested genotypes, loci Wx-A1 and Wx-D1 were amplified in all genotypes. Percentage of mealy caryopses per sample ranged from 0 to 100 % among tested genotypes. No significant correlation between these two parameters was observed. (en)
Title
  • Granule-bound starch synthase and endosperm mealiness correlaton in wheat (Triticum aestivum L.) with nonstandard colored caryopses
  • Granule-bound starch synthase and endosperm mealiness correlaton in wheat (Triticum aestivum L.) with nonstandard colored caryopses (en)
skos:prefLabel
  • Granule-bound starch synthase and endosperm mealiness correlaton in wheat (Triticum aestivum L.) with nonstandard colored caryopses
  • Granule-bound starch synthase and endosperm mealiness correlaton in wheat (Triticum aestivum L.) with nonstandard colored caryopses (en)
skos:notation
  • RIV/62156489:43210/14:00229014!RIV15-MSM-43210___
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  • 18561
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  • RIV/62156489:43210/14:00229014
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  • mealy endosperm; Triticum aestivum L.; waxy protein; wheat; GBBS (en)
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  • [FFBC5587FFEE]
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  • Brno, Czech Republic
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  • Brno, Czech Republic
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  • MendelNet 2014 - Proceedings of International PhD Students Conference
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  • Havel, Ladislav
  • Mrkvicová, Eva
  • Trojan, Václav
  • Vyhnánek, Tomáš
  • Hřivna, Luděk
  • Štiasna, Klára
  • Presinszká, Mária
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  • Mendel University in Brno
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  • 978-80-7509-174-1
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  • 43210
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