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  • Accumulation of secondary metabolites (general phenols, naphthodianthrones and phloroglucinol hyperforin) in Hypericum perforatum and Hypericum canariense after application of the inhibitor (2-aminoindane-2-phosphonic acid, AIP) of the pivotal enzyme of general phenylpropanoid pathway (phenylalanine ammonia-lyase, PAL) was studied. Shoots of H. perforatum revealed more expressive growth depression, concomitantly with the inhibition of PAL activity (-60%) and decrease in soluble phenols and individual phenolic acids in response to AIP. Hypericins (hypericin, pseudohypericin and protohypericin) decreased while hyperforin increased in AIP-cultured H. perforatum. On the contrary, growth changes, decreases in soluble phenols and individual phenolic acids were less-visible in H. canariense. This was also reflected in restoration of PAL activity (+330%) and selected flavonoids even increased. Hypericins and hyperforin were present in several orders of magnitude lower amounts in comparison with H. perforatum. Increase in proline indicates potential compensatory antioxidative mechanism if phenols are depleted. Microscopy revealed also differences in secondary xylem formation and lignification between species after exposure to AIP. (c) 2012 Elsevier Masson SAS. All rights reserved.
  • Accumulation of secondary metabolites (general phenols, naphthodianthrones and phloroglucinol hyperforin) in Hypericum perforatum and Hypericum canariense after application of the inhibitor (2-aminoindane-2-phosphonic acid, AIP) of the pivotal enzyme of general phenylpropanoid pathway (phenylalanine ammonia-lyase, PAL) was studied. Shoots of H. perforatum revealed more expressive growth depression, concomitantly with the inhibition of PAL activity (-60%) and decrease in soluble phenols and individual phenolic acids in response to AIP. Hypericins (hypericin, pseudohypericin and protohypericin) decreased while hyperforin increased in AIP-cultured H. perforatum. On the contrary, growth changes, decreases in soluble phenols and individual phenolic acids were less-visible in H. canariense. This was also reflected in restoration of PAL activity (+330%) and selected flavonoids even increased. Hypericins and hyperforin were present in several orders of magnitude lower amounts in comparison with H. perforatum. Increase in proline indicates potential compensatory antioxidative mechanism if phenols are depleted. Microscopy revealed also differences in secondary xylem formation and lignification between species after exposure to AIP. (c) 2012 Elsevier Masson SAS. All rights reserved. (en)
Title
  • PAL inhibitor evokes different responses in two Hypericum species
  • PAL inhibitor evokes different responses in two Hypericum species (en)
skos:prefLabel
  • PAL inhibitor evokes different responses in two Hypericum species
  • PAL inhibitor evokes different responses in two Hypericum species (en)
skos:notation
  • RIV/62156489:43210/13:00218796!RIV15-MSM-43210___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • I, P(EE2.3.30.0017)
http://linked.open...iv/cisloPeriodika
  • February 2013
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 95149
http://linked.open...ai/riv/idVysledku
  • RIV/62156489:43210/13:00218796
http://linked.open...riv/jazykVysledku
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  • phenolic metabolism; chlorogenic acid; flavonols; lignin; free amino acids (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • FR - Francouzská republika
http://linked.open...ontrolniKodProRIV
  • [559607689A8F]
http://linked.open...i/riv/nazevZdroje
  • Plant Physiology and Biochemistry
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
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http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 63
http://linked.open...iv/tvurceVysledku
  • Babula, Petr
  • Klejdus, Bořivoj
  • Kováčik, Jozef
http://linked.open...ain/vavai/riv/wos
  • 315011400011
issn
  • 0981-9428
number of pages
http://bibframe.org/vocab/doi
  • 10.1016/j.plaphy.2012.11.019
http://localhost/t...ganizacniJednotka
  • 43210
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