About: Changes of metal-induced toxicity by H2O2/NO modulators in Scenedesmus quadricauda (Chlorophyceae)     Goto   Sponge   NotDistinct   Permalink

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  • Effect of nitric oxide donor (sodium nitroprusside, SNP, 500 mu M) or hydrogen peroxide scavenger (dithiothreitol, DTT, 500 mu M) on cadmium (Cd) or copper (Cu) uptake (150 mu M solutions) and toxicity using Scenedesmus quadricauda was studied. Combined treatments (Cd or Cu + DTT or SNP) usually ameliorated metal-induced toxicity at the level of pigments, proteins, and mineral nutrients in comparison with respective metal alone. Viability tests (MTT and TTC) showed the lowest values preferentially in Cu treatments, indicating higher toxicity in comparison with Cd. Cd showed low impact on amino acids while strong Cu-induced depletion was mitigated by DTT and SNP. Amount of ROS and NO showed the most pronounced responses in SNP variants being rather reciprocal than parallel and regulated ascorbate peroxidase activity. Blot gel analyses of hsp70 protein did not reveal extensive changes after given exposure period. Phenols were elevated by DTT alone while all Cu treatments revealed depletion. Total Cu content decreased while total Cd content increased in metal + SNP or metal + DTT. Subsequent experiment using lower Cd, SNP or DTT doses (10 and 100 mu M) revealed concentration-dependent impact on Cd uptake. Overall, DTT was found to be more suitable for the amelioration of metal-induced toxicity.
  • Effect of nitric oxide donor (sodium nitroprusside, SNP, 500 mu M) or hydrogen peroxide scavenger (dithiothreitol, DTT, 500 mu M) on cadmium (Cd) or copper (Cu) uptake (150 mu M solutions) and toxicity using Scenedesmus quadricauda was studied. Combined treatments (Cd or Cu + DTT or SNP) usually ameliorated metal-induced toxicity at the level of pigments, proteins, and mineral nutrients in comparison with respective metal alone. Viability tests (MTT and TTC) showed the lowest values preferentially in Cu treatments, indicating higher toxicity in comparison with Cd. Cd showed low impact on amino acids while strong Cu-induced depletion was mitigated by DTT and SNP. Amount of ROS and NO showed the most pronounced responses in SNP variants being rather reciprocal than parallel and regulated ascorbate peroxidase activity. Blot gel analyses of hsp70 protein did not reveal extensive changes after given exposure period. Phenols were elevated by DTT alone while all Cu treatments revealed depletion. Total Cu content decreased while total Cd content increased in metal + SNP or metal + DTT. Subsequent experiment using lower Cd, SNP or DTT doses (10 and 100 mu M) revealed concentration-dependent impact on Cd uptake. Overall, DTT was found to be more suitable for the amelioration of metal-induced toxicity. (en)
Title
  • Changes of metal-induced toxicity by H2O2/NO modulators in Scenedesmus quadricauda (Chlorophyceae)
  • Changes of metal-induced toxicity by H2O2/NO modulators in Scenedesmus quadricauda (Chlorophyceae) (en)
skos:prefLabel
  • Changes of metal-induced toxicity by H2O2/NO modulators in Scenedesmus quadricauda (Chlorophyceae)
  • Changes of metal-induced toxicity by H2O2/NO modulators in Scenedesmus quadricauda (Chlorophyceae) (en)
skos:notation
  • RIV/62156489:43210/13:00210886!RIV14-MSM-43210___
http://linked.open...avai/riv/aktivita
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  • P(ED1.1.00/02.0068)
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  • 8
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  • 64962
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  • RIV/62156489:43210/13:00210886
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  • oxidační stres; Matricaria chamomilla; Chlamydomonas reinhardtii; těžké kovy; měď; Nitric oxide (en)
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  • DE - Spolková republika Německo
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  • [7D7962FD1565]
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  • Environmental Science and Pollution Research
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  • 20
http://linked.open...iv/tvurceVysledku
  • Klejdus, Bořivoj
  • Hedbávný, Josef
  • Kováčik, Jozef
  • Štork, František
  • Báčkor, Martin
issn
  • 0944-1344
number of pages
http://bibframe.org/vocab/doi
  • 10.1007/s11356-013-1541-0
http://localhost/t...ganizacniJednotka
  • 43210
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