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Description
| - The aim of this study was to evaluate the effect of age on sperm activity, sperm cell viability and total number of spermatozoa in the ejaculate of dogs. We evaluated 90 semen samples. The dogs were divided into groups according to the age (A: 1.5--2 years, B: 2--5 years, C: 5--6.5 years). Semen samples were collected by manual manipulation into the glass beaker. Immediately after collection of macroscopic examination was made for all samples, which included find out volume of ejaculate, sperm activity, concentration and sperm cell viability. Volume was measured using the graduated cylinder. Concentration was evaluated by haematocytometry method using Bürker chamber and activity by subjective method according to the percentage of motile sperm in the native ejaculate. We evaluated the percentage of sperm with progressive direct movement after the head. Viability eosin-nigrosin stain method was performed for evaluation. In this case, we evaluated the total number of alive and dead sperms. Monitored characteristics were expressed in weighted average and standard error. Based on the results we can state that, in case of monitoring factors, the age of dogs had the significant influence. In case of activity, as well as viability, statistically highly significant differences (P < 0.01) were observed between group of youngest dogs (A: 1.5--2 years) and oldest dogs (C: 5--6.5 years). Statistically significant difference (P < 0.05) was proved between dogs from group B (2--5 years) and dogs from group C (5--6.5 years). In conclusion, the negative correlation of age, in case of activity (r = -0.44; P < 0.001) and even viability (r = -0.33; P < 0.01), was demonstrated. With increasing age, the values of both factors were reduced. In case of total number of spermatozoa, this phenomenon was not observed (r = 0.01; P > 0.05).
- The aim of this study was to evaluate the effect of age on sperm activity, sperm cell viability and total number of spermatozoa in the ejaculate of dogs. We evaluated 90 semen samples. The dogs were divided into groups according to the age (A: 1.5--2 years, B: 2--5 years, C: 5--6.5 years). Semen samples were collected by manual manipulation into the glass beaker. Immediately after collection of macroscopic examination was made for all samples, which included find out volume of ejaculate, sperm activity, concentration and sperm cell viability. Volume was measured using the graduated cylinder. Concentration was evaluated by haematocytometry method using Bürker chamber and activity by subjective method according to the percentage of motile sperm in the native ejaculate. We evaluated the percentage of sperm with progressive direct movement after the head. Viability eosin-nigrosin stain method was performed for evaluation. In this case, we evaluated the total number of alive and dead sperms. Monitored characteristics were expressed in weighted average and standard error. Based on the results we can state that, in case of monitoring factors, the age of dogs had the significant influence. In case of activity, as well as viability, statistically highly significant differences (P < 0.01) were observed between group of youngest dogs (A: 1.5--2 years) and oldest dogs (C: 5--6.5 years). Statistically significant difference (P < 0.05) was proved between dogs from group B (2--5 years) and dogs from group C (5--6.5 years). In conclusion, the negative correlation of age, in case of activity (r = -0.44; P < 0.001) and even viability (r = -0.33; P < 0.01), was demonstrated. With increasing age, the values of both factors were reduced. In case of total number of spermatozoa, this phenomenon was not observed (r = 0.01; P > 0.05). (en)
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Title
| - Effect of age on the sperm activity, sperm cell viability and total number of spermatozoa in the ejaculate of dogs
- Effect of age on the sperm activity, sperm cell viability and total number of spermatozoa in the ejaculate of dogs (en)
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skos:prefLabel
| - Effect of age on the sperm activity, sperm cell viability and total number of spermatozoa in the ejaculate of dogs
- Effect of age on the sperm activity, sperm cell viability and total number of spermatozoa in the ejaculate of dogs (en)
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skos:notation
| - RIV/62156489:43210/13:00210092!RIV14-MSM-43210___
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/62156489:43210/13:00210092
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - dog ejaculate; age; sperm cell viability; total number of spermatozoa; sperm activity (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...ontrolniKodProRIV
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http://linked.open...v/mistoKonaniAkce
| - Mendelova univerzita v Brně
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http://linked.open...i/riv/mistoVydani
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http://linked.open...i/riv/nazevZdroje
| - MendelNet 2013 - Proceedings of International PhD Students Conference
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...UplatneniVysledku
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http://linked.open...iv/tvurceVysledku
| - Filipčík, Radek
- Hošek, Martin
- Máchal, Ladislav
- Paldusová, Michaela
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http://linked.open...vavai/riv/typAkce
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http://linked.open.../riv/zahajeniAkce
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number of pages
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http://purl.org/ne...btex#hasPublisher
| - Mendel University in Brno, Zemedelska 1, 613 00 Brno, Czech Republic
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https://schema.org/isbn
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http://localhost/t...ganizacniJednotka
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