| Attributes | Values |
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| rdf:type
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| Description
| - Anthocyanins are responsible for the coloration in shades of blue, purple and red of various body parts of plants. Wheat caryopses with nonstandard coloration, specifically genotypes UC66049 and Tschermaks Blaukörniger Sommerweizen with blue aleurone, ANK28B and Abyssinskaya arraseita with purple pericarp were used in the experiment. Genotype Novosibirskaya 67 was used as a standard because it does not synthesize any pigments. Total RNA was isolated from caryopses by the phenol-chloroform method and transcribed into cDNA. In dihydroflavonol reductase (DFR) gene sequences obtained from direct sequencing of PCR product were detected several indels and single nucleotide polymorphisms. The similarity among all sequences of analyzed genotypes and sequence obtained from National Center for Biotechnology Information (NCBI) ranged between 94.59 to 100 %. The gene expression of DFR in samples varied during maturation. For the control genotype Novosibirskaya 67 there was highest DFR gene expression 25 days post anthesis (dpa) and the lowest 15 and 35 dpa. In Tschermaks Blaukörniger Sommerweizen was observed the lowest gene expression 40 dpa and the highest 10 dpa, while in genotype UC66049 was DFR gene expression almost the lowest 10 dpa and 40 dpa reached the lowest values. The highest DFR gene expression in Abyssinskaya arraseita was observed between 15 and 20 dpa and then rapidly decreases to its lowest level 25 dpa. Genotype ANK28B shows decreasing gene expression during maturation, the highest value was observed 10 dpa and the lowest 35 dpa, 40 dpa gene expression slightly increased again.
- Anthocyanins are responsible for the coloration in shades of blue, purple and red of various body parts of plants. Wheat caryopses with nonstandard coloration, specifically genotypes UC66049 and Tschermaks Blaukörniger Sommerweizen with blue aleurone, ANK28B and Abyssinskaya arraseita with purple pericarp were used in the experiment. Genotype Novosibirskaya 67 was used as a standard because it does not synthesize any pigments. Total RNA was isolated from caryopses by the phenol-chloroform method and transcribed into cDNA. In dihydroflavonol reductase (DFR) gene sequences obtained from direct sequencing of PCR product were detected several indels and single nucleotide polymorphisms. The similarity among all sequences of analyzed genotypes and sequence obtained from National Center for Biotechnology Information (NCBI) ranged between 94.59 to 100 %. The gene expression of DFR in samples varied during maturation. For the control genotype Novosibirskaya 67 there was highest DFR gene expression 25 days post anthesis (dpa) and the lowest 15 and 35 dpa. In Tschermaks Blaukörniger Sommerweizen was observed the lowest gene expression 40 dpa and the highest 10 dpa, while in genotype UC66049 was DFR gene expression almost the lowest 10 dpa and 40 dpa reached the lowest values. The highest DFR gene expression in Abyssinskaya arraseita was observed between 15 and 20 dpa and then rapidly decreases to its lowest level 25 dpa. Genotype ANK28B shows decreasing gene expression during maturation, the highest value was observed 10 dpa and the lowest 35 dpa, 40 dpa gene expression slightly increased again. (en)
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| Title
| - Genomic analyses of dihydroflavonol reductase gene in genotypes of common wheat (Triticum aestivum L.) with nonstandard coloured caryopses
- Genomic analyses of dihydroflavonol reductase gene in genotypes of common wheat (Triticum aestivum L.) with nonstandard coloured caryopses (en)
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| skos:prefLabel
| - Genomic analyses of dihydroflavonol reductase gene in genotypes of common wheat (Triticum aestivum L.) with nonstandard coloured caryopses
- Genomic analyses of dihydroflavonol reductase gene in genotypes of common wheat (Triticum aestivum L.) with nonstandard coloured caryopses (en)
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| skos:notation
| - RIV/62156489:43210/13:00209872!RIV14-MSM-43210___
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/62156489:43210/13:00209872
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - wheat; dihydroflavonol reductase; genomic analysis (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...v/mistoKonaniAkce
| - Mendelova univerzita v Brně
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| http://linked.open...i/riv/mistoVydani
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| http://linked.open...i/riv/nazevZdroje
| - MendelNet 2013 - Proceedings of International PhD Students Conference
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| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...UplatneniVysledku
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| http://linked.open...iv/tvurceVysledku
| - Bartoš, Jan
- Havel, Ladislav
- Trojan, Václav
- Vyhnánek, Tomáš
- Sekanina, Petr
- Štiasna, Klára
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| http://linked.open...vavai/riv/typAkce
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| http://linked.open.../riv/zahajeniAkce
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| number of pages
| |
| http://purl.org/ne...btex#hasPublisher
| - Mendel University in Brno, Zemedelska 1, 613 00 Brno, Czech Republic
|
| https://schema.org/isbn
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| http://localhost/t...ganizacniJednotka
| |
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