About: Study of chalcon isomerase and anthocyanidin synthase DNA sequences in wheat (Triticum aestivum L.)     Goto   Sponge   NotDistinct   Permalink

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  • Anthocyanins, a group of flavonoid substances that is responsible for colored caryopses of common wheat (Triticum aestivum L.). Pigments formed in anthocyanin biosynthetic pathway are deposited in different parts of the caryopsis. Purple anthocyanins are accumulated in pericarp while blue anthocyanins are stored in aleurone layer of caryopsis. The spring wheat form of two genotypes with purple pericarp (Abyssinskaya arraseita and ANK-28B) and two genotypes with blue aleuron layer (Tschermaks Blaukörniger Sommerweizen and UC66049) were used in the experiments. Genotype with white caryopsis (Novosibirskaya 67) was used as a control. Total RNA was isolated from developing caryopses and transcribed into cDNA. Sequences for chalcon isomerase (98-100% similarity) and for anthocyanidin synthase genes were detected. The variability among genotypes in this study was due to insertion or deletion (indels). These candidate sequences were localized in the wheat genome and will be used for study of gene expression during maturation.
  • Anthocyanins, a group of flavonoid substances that is responsible for colored caryopses of common wheat (Triticum aestivum L.). Pigments formed in anthocyanin biosynthetic pathway are deposited in different parts of the caryopsis. Purple anthocyanins are accumulated in pericarp while blue anthocyanins are stored in aleurone layer of caryopsis. The spring wheat form of two genotypes with purple pericarp (Abyssinskaya arraseita and ANK-28B) and two genotypes with blue aleuron layer (Tschermaks Blaukörniger Sommerweizen and UC66049) were used in the experiments. Genotype with white caryopsis (Novosibirskaya 67) was used as a control. Total RNA was isolated from developing caryopses and transcribed into cDNA. Sequences for chalcon isomerase (98-100% similarity) and for anthocyanidin synthase genes were detected. The variability among genotypes in this study was due to insertion or deletion (indels). These candidate sequences were localized in the wheat genome and will be used for study of gene expression during maturation. (en)
Title
  • Study of chalcon isomerase and anthocyanidin synthase DNA sequences in wheat (Triticum aestivum L.)
  • Study of chalcon isomerase and anthocyanidin synthase DNA sequences in wheat (Triticum aestivum L.) (en)
skos:prefLabel
  • Study of chalcon isomerase and anthocyanidin synthase DNA sequences in wheat (Triticum aestivum L.)
  • Study of chalcon isomerase and anthocyanidin synthase DNA sequences in wheat (Triticum aestivum L.) (en)
skos:notation
  • RIV/62156489:43210/13:00209871!RIV14-MSM-43210___
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  • 108659
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  • RIV/62156489:43210/13:00209871
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  • wheat; anthocyanidin synthase; chalcone isomerase (en)
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  • [31A2E4EAC06E]
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  • Mendelova univerzita v Brně
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  • Faculty of Agronomy
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  • MendelNet 2013 - Proceedings of International PhD Students Conference
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  • Bartoš, Jan
  • Havel, Ladislav
  • Trojan, Václav
  • Vyhnánek, Tomáš
  • Sekanina, Petr
  • Štiasna, Klára
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  • Mendel University in Brno, Zemedelska 1, 613 00 Brno, Czech Republic
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  • 978-80-7375-908-7
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  • 43210
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