About: A determination of oxidative stress in tumour cells treated with cytostatics     Goto   Sponge   NotDistinct   Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

AttributesValues
rdf:type
Description
  • Oxidative stress as a response to anticancer drugs is not sufficiently investigated and direct measurement of reactive oxygen species and oxidative stress markers in clinical medicine is still difficult [1]. In our study, oxidative status was studied in model tumour cell lines derived from tumours of the prostate (PC-3, and PNT1A 22RV1). Cell lines were cultured with anticancer drugs carboplatin, cisplatin, oxaliplatin at concentrations of 0, 10, 30, 50, 80, 100, 150; 180; 200 and 250 umol*l-1 and drugs as doxorubicin and ellipticine at concentrations of 0, 0.5, 1, 1.5, 2, 4, 6, 10, 12, 15 umol*l-1. The rate of oxidative stress was determined by antioxidant activity (FRAP, DPPH and ABTS). Based on the results obtained from oxidative changes in the cells dtermined by FRAP and ABTS, it was found the lowest antioxidant activity as 0.06 mg of Trolox*l-1 in cell lines 22RV1 treated with carboplatin at a concentration of 100 umol*l-1. PNT1A cells gave the lowest antioxidant activity of 0.35 mg of Trolox*l-1 after exposure to cisplatin concentration of 100 umol*l-1.
  • Oxidative stress as a response to anticancer drugs is not sufficiently investigated and direct measurement of reactive oxygen species and oxidative stress markers in clinical medicine is still difficult [1]. In our study, oxidative status was studied in model tumour cell lines derived from tumours of the prostate (PC-3, and PNT1A 22RV1). Cell lines were cultured with anticancer drugs carboplatin, cisplatin, oxaliplatin at concentrations of 0, 10, 30, 50, 80, 100, 150; 180; 200 and 250 umol*l-1 and drugs as doxorubicin and ellipticine at concentrations of 0, 0.5, 1, 1.5, 2, 4, 6, 10, 12, 15 umol*l-1. The rate of oxidative stress was determined by antioxidant activity (FRAP, DPPH and ABTS). Based on the results obtained from oxidative changes in the cells dtermined by FRAP and ABTS, it was found the lowest antioxidant activity as 0.06 mg of Trolox*l-1 in cell lines 22RV1 treated with carboplatin at a concentration of 100 umol*l-1. PNT1A cells gave the lowest antioxidant activity of 0.35 mg of Trolox*l-1 after exposure to cisplatin concentration of 100 umol*l-1. (en)
Title
  • A determination of oxidative stress in tumour cells treated with cytostatics
  • A determination of oxidative stress in tumour cells treated with cytostatics (en)
skos:prefLabel
  • A determination of oxidative stress in tumour cells treated with cytostatics
  • A determination of oxidative stress in tumour cells treated with cytostatics (en)
skos:notation
  • RIV/62156489:43210/12:00198423!RIV13-GA0-43210___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GAP301/10/0356)
http://linked.open...iv/cisloPeriodika
  • 1
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 120164
http://linked.open...ai/riv/idVysledku
  • RIV/62156489:43210/12:00198423
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • oxidative stress; cytostatics; antioxidant activity (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • GR - Řecká republika
http://linked.open...ontrolniKodProRIV
  • [3F80D362145D]
http://linked.open...i/riv/nazevZdroje
  • International Journal of Molecular Medicine
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 30
http://linked.open...iv/tvurceVysledku
  • Adam, Vojtěch
  • Eckschlager, Tomáš
  • Kizek, René
  • Křížková, Soňa
  • Masařík, Michal
  • Sochor, Jiří
  • Stiborová, Marie
  • Ruttkay-Nedecký, Branislav
issn
  • 1107-3756
number of pages
http://localhost/t...ganizacniJednotka
  • 43210
Faceted Search & Find service v1.16.118 as of Jun 21 2024


Alternative Linked Data Documents: ODE     Content Formats:   [cxml] [csv]     RDF   [text] [turtle] [ld+json] [rdf+json] [rdf+xml]     ODATA   [atom+xml] [odata+json]     Microdata   [microdata+json] [html]    About   
This material is Open Knowledge   W3C Semantic Web Technology [RDF Data] Valid XHTML + RDFa
OpenLink Virtuoso version 07.20.3240 as of Jun 21 2024, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (126 GB total memory, 48 GB memory in use)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software