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Description
| - In food-grade soybeans for human nutrition, sucrose content is a character of interest apart from protein, because sucrose affects flavour and other soy-food properties. As determination of sucrose content through separative or enzymatic methods is not suitable for analysing large numbers of samples, the objective of this research was to develop near-infrared reflectance spectroscopy (NIRS) calibrations for rapid screening of breeding lines for sucrose. Conventional soybean samples have a sucrose content of 30--60 g/kg, whereas high-sucrose reference samples are lacking. Therefore, both natural and artificial (additional sucrose added) reference samples with a sucrose content of up to 140 g/ kg were combined, and a NIRS calibration model was developed (cross-validation R2 = 0.969) for sucrose. Utilizing wavelength ranges with homogeneous performance in both natural and artificial samples, a prediction model was obtained, which suggests a stable performance of the calibration over a wide range of sucrose concentrations. Thus, identification of high-sucrose soybean introgressions should be possible in appropriate segregating populations.
- In food-grade soybeans for human nutrition, sucrose content is a character of interest apart from protein, because sucrose affects flavour and other soy-food properties. As determination of sucrose content through separative or enzymatic methods is not suitable for analysing large numbers of samples, the objective of this research was to develop near-infrared reflectance spectroscopy (NIRS) calibrations for rapid screening of breeding lines for sucrose. Conventional soybean samples have a sucrose content of 30--60 g/kg, whereas high-sucrose reference samples are lacking. Therefore, both natural and artificial (additional sucrose added) reference samples with a sucrose content of up to 140 g/ kg were combined, and a NIRS calibration model was developed (cross-validation R2 = 0.969) for sucrose. Utilizing wavelength ranges with homogeneous performance in both natural and artificial samples, a prediction model was obtained, which suggests a stable performance of the calibration over a wide range of sucrose concentrations. Thus, identification of high-sucrose soybean introgressions should be possible in appropriate segregating populations. (en)
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Title
| - Near-infrared reflectance calibrations for determining sucrose content in soybean breeding using artificial reference samples
- Near-infrared reflectance calibrations for determining sucrose content in soybean breeding using artificial reference samples (en)
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skos:prefLabel
| - Near-infrared reflectance calibrations for determining sucrose content in soybean breeding using artificial reference samples
- Near-infrared reflectance calibrations for determining sucrose content in soybean breeding using artificial reference samples (en)
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skos:notation
| - RIV/62156489:43210/12:00188047!RIV13-MSM-43210___
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/62156489:43210/12:00188047
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - seed quality; sucrose content; near infrared reflectance spectroscopy; soybean (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
| - DE - Spolková republika Německo
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...UplatneniVysledku
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http://linked.open...iv/tvurceVysledku
| - Lošák, Tomáš
- Vollmann, Johann
- Berghofer, Emmerich
- Sato, Takashi
- Zahlner, Viola
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http://localhost/t...ganizacniJednotka
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