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Description
  • Polycyclic aromatic hydrocarbons (PAHs) belong to the group of the most important pollutants of the living environment, which are present in air, soils, freshwater, seawater and sediments. They have very substantial effects on all living organisms including plants and animals. Plants represent important point in PAHs food chain entry. Despite the fact that PAHs influence on animals is intensively studied, effect on plants is almost unknown. In our study, action of polycyclic aromatic hydrocarbon fluoranthene on a plant cell experimental model -- tobacco BY-2 cell suspension culture -- was studied. BY-2 cells were exposed to the fluoranthene in concentration range from 0 to 1000 _M, duration of treatment was 120 h, respectively 5 days. Samples were collected in the strictly defined time intervals of 24 h. Exposure of the BY-2 cells led to significant changes in viability, changes in autofluorescence due to accumulation of fluoranthene in lipophilic cell compartments, especially biomembranes, and production of reactive oxygen species, which resulted in damage of biomembranes and disruption of their semipermeability and initiation of process of programmed cell death. Obtained results bring new knowledge about phytotoxicity of fluoranthene and mechanism of its action.
  • Polycyclic aromatic hydrocarbons (PAHs) belong to the group of the most important pollutants of the living environment, which are present in air, soils, freshwater, seawater and sediments. They have very substantial effects on all living organisms including plants and animals. Plants represent important point in PAHs food chain entry. Despite the fact that PAHs influence on animals is intensively studied, effect on plants is almost unknown. In our study, action of polycyclic aromatic hydrocarbon fluoranthene on a plant cell experimental model -- tobacco BY-2 cell suspension culture -- was studied. BY-2 cells were exposed to the fluoranthene in concentration range from 0 to 1000 _M, duration of treatment was 120 h, respectively 5 days. Samples were collected in the strictly defined time intervals of 24 h. Exposure of the BY-2 cells led to significant changes in viability, changes in autofluorescence due to accumulation of fluoranthene in lipophilic cell compartments, especially biomembranes, and production of reactive oxygen species, which resulted in damage of biomembranes and disruption of their semipermeability and initiation of process of programmed cell death. Obtained results bring new knowledge about phytotoxicity of fluoranthene and mechanism of its action. (en)
Title
  • Effect of fluoranthene on plant cell model: Tobacco BY-2 suspension culture
  • Effect of fluoranthene on plant cell model: Tobacco BY-2 suspension culture (en)
skos:prefLabel
  • Effect of fluoranthene on plant cell model: Tobacco BY-2 suspension culture
  • Effect of fluoranthene on plant cell model: Tobacco BY-2 suspension culture (en)
skos:notation
  • RIV/62156489:43210/12:00183145!RIV15-GA0-43210___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GA522/09/0239), P(GD102/09/H083), S, V, Z(MSM6215712402)
http://linked.open...iv/cisloPeriodika
  • May 2012
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 133050
http://linked.open...ai/riv/idVysledku
  • RIV/62156489:43210/12:00183145
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • plant cell; fluoranthene; suspension culture (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • NL - Nizozemsko
http://linked.open...ontrolniKodProRIV
  • [79C1261B2027]
http://linked.open...i/riv/nazevZdroje
  • Environmental and Experimental Botany
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 78
http://linked.open...iv/tvurceVysledku
  • Adam, Vojtěch
  • Beklová, Miroslava
  • Babula, Petr
  • Havel, Ladislav
  • Kizek, René
  • Kummerová, Marie
  • Provazník, Ivo
  • Škutková, Helena
  • Hošek, J.
  • Vodička, Ondřej
http://linked.open...ain/vavai/riv/wos
  • 301685000016
http://linked.open...n/vavai/riv/zamer
issn
  • 0098-8472
number of pages
http://bibframe.org/vocab/doi
  • 10.1016/j.envexpbot.2011.12.024
http://localhost/t...ganizacniJednotka
  • 43210
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