About: Gas chromatographic-mas spectrometric determination of low molecular products formed during exposition of polycaprolactone to aspergillus-lipase

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About: Gas chromatographic-mas spectrometric determination of low molecular products formed during exposition of polycaprolactone to aspergillus-lipase Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://www.chemicke-listy.cz/docs/full/2011_18_s871-s1072.pdf
Description	This work reports on the determination of possible degradation products released from PCL films aged in the presence of lipase-type enzyme by means of the gas chromatographic-mass spectrometric analysis. The analysis was performed on HP-6890 gas chromatograph equipped with the mass spectrometric detector HP-5673. HP-5MS column (30 m x 0,25 mm x 0,5 mikm, film of bonded 5 % phenyl, 95 % dimethylpolysiloxane, Hewlett-Packard, Waldbronn, Germany) was used. PCL substrate in the film form (Mn = 19 300 g/mol) was added to a phosphate buffer system with pH = 7 containing 0,05 mg ml-1 Aspergillus sp. lipase with the initial enzymatic activity of 0,3 mmol min-1 ml-1. The degradation experiments were carried out for 42 days at 37 °C. For analyses purpose, the aliquots of buffer solution, used in degradation tests, were withdrawn in certain intervals and extracted with dichloromethane. Subsequently, the presence of e-caprolactone was confirmed in the extracts. The decrease in the amount of e-caprolactone (18,1 -- 0,65 mikg.g-1) was observed during the degradation period. This work reports on the determination of possible degradation products released from PCL films aged in the presence of lipase-type enzyme by means of the gas chromatographic-mass spectrometric analysis. The analysis was performed on HP-6890 gas chromatograph equipped with the mass spectrometric detector HP-5673. HP-5MS column (30 m x 0,25 mm x 0,5 mikm, film of bonded 5 % phenyl, 95 % dimethylpolysiloxane, Hewlett-Packard, Waldbronn, Germany) was used. PCL substrate in the film form (Mn = 19 300 g/mol) was added to a phosphate buffer system with pH = 7 containing 0,05 mg ml-1 Aspergillus sp. lipase with the initial enzymatic activity of 0,3 mmol min-1 ml-1. The degradation experiments were carried out for 42 days at 37 °C. For analyses purpose, the aliquots of buffer solution, used in degradation tests, were withdrawn in certain intervals and extracted with dichloromethane. Subsequently, the presence of e-caprolactone was confirmed in the extracts. The decrease in the amount of e-caprolactone (18,1 -- 0,65 mikg.g-1) was observed during the degradation period. (en)
Title	Gas chromatographic-mas spectrometric determination of low molecular products formed during exposition of polycaprolactone to aspergillus-lipase Gas chromatographic-mas spectrometric determination of low molecular products formed during exposition of polycaprolactone to aspergillus-lipase (en)
skos:prefLabel	Gas chromatographic-mas spectrometric determination of low molecular products formed during exposition of polycaprolactone to aspergillus-lipase Gas chromatographic-mas spectrometric determination of low molecular products formed during exposition of polycaprolactone to aspergillus-lipase (en)
skos:notation	RIV/62156489:43210/11:00182658!RIV12-MSM-43210___
http://linked.open...avai/riv/aktivita	S
http://linked.open...avai/riv/aktivity	S
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Voběrková, Stanislava Helánová, Valerie Kulštejnová, Lucie
http://linked.open.../riv/druhVysledku	A(V) - Audiovizuální tvorba se vzdáleným přístupem
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Mendelova univerzita v Brně / Agronomická fakulta
http://linked.open...dnocenehoVysledku	200810
http://linked.open...ai/riv/idVysledku	RIV/62156489:43210/11:00182658
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	PCL; gas chromatography - mass spectrometry (gc-ms); polycaprolactone; analysis (en)
http://linked.open.../riv/klicoveSlovo	polycaprolactone PCL analysis gas chromatography
http://linked.open...i/riv/kodPristupu	V
http://linked.open...ontrolniKodProRIV	[09EE9B6A729E]
http://linked.open...in/vavai/riv/obor	CB
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...UplatneniVysledku	2011
http://linked.open...iv/tvurceVysledku	Omelková, Jiřina Hermanová, Soňa Voběrková, Stanislava Helánová, Valerie Kulštejnová, Lucie
http://localhost/t...ganizacniJednotka	43210

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