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| Description
| - Extraction of anthocyanins from pigmented wheat grains and their analysis using HPLC/MS is described. Grain from six wheat lines differing in pigmentation was used in this study. Wheat grains were harvested every five days beginning 15th day post anthesis, ending once the grains reached physiologic maturity (40th day post anthesis), for a total six sampling dates. Anthocyanins were extracted with 10 ml acidified methanol (methanol and 1, 0 N HCl, 85:15 v/v) and shaking on Ika Ultra- Turrax Tube Drive, for 2x29 min. The filtered extract were evaporated at 50 °C to dryness and redissolved in acidifield methanol. Individual anthocyanins were separated and quantified by high-performance liquid chromatography (HPLC). Anthocyanin concentration increased rapidly during grain development and then decreased before maturity. Cyanidin-3- glucoside was the predominant anthocyanin in purple wheat. Delphinidin-3-glucoside is the principal one in blue wheat. Anthocyanins are considered to be physiologically active substances, whose importance in promoting health and reducing the risk of chronic disease has been documented. This means that the presence of wheat anthocyanins in pigmented wheat, can be a source of bioactive compounds for use in the food industry
- Extraction of anthocyanins from pigmented wheat grains and their analysis using HPLC/MS is described. Grain from six wheat lines differing in pigmentation was used in this study. Wheat grains were harvested every five days beginning 15th day post anthesis, ending once the grains reached physiologic maturity (40th day post anthesis), for a total six sampling dates. Anthocyanins were extracted with 10 ml acidified methanol (methanol and 1, 0 N HCl, 85:15 v/v) and shaking on Ika Ultra- Turrax Tube Drive, for 2x29 min. The filtered extract were evaporated at 50 °C to dryness and redissolved in acidifield methanol. Individual anthocyanins were separated and quantified by high-performance liquid chromatography (HPLC). Anthocyanin concentration increased rapidly during grain development and then decreased before maturity. Cyanidin-3- glucoside was the predominant anthocyanin in purple wheat. Delphinidin-3-glucoside is the principal one in blue wheat. Anthocyanins are considered to be physiologically active substances, whose importance in promoting health and reducing the risk of chronic disease has been documented. This means that the presence of wheat anthocyanins in pigmented wheat, can be a source of bioactive compounds for use in the food industry (cs)
- Extrakce antokyanů z pigmentových pšeničná zrna a jejich analýzu pomocí HPLC / MS je popsána. Zrno od šesti pšenice linky se liší v pigmentaci byla použita v této studii. Pšeničná zrna byly sklizeny za pět dní počínaje 15. dnem po kvetení, končí po dosažení fyziologické zralosti zrna (40. den po kvetení), pro celkem šest odběry. Antokyany byly extrahovány s 10 ml metanolu okyseleného (methanol, 1, 0 N HCl, 85:15 V / V) a třásl na Ika Ultra- Turrax Tube Drive, na 2x29 min. Filtrovaný výtažek se odpaří při 50 ° C do sucha a rozpustí ve acidifield methanol. Individuální antokyanů byli odděleni a kvantifikovat vysoce účinné kapalinové chromatografie (HPLC). Anthokyaninového zvýšila koncentrace rychle během vývoje zrna a poté snížil před splatností. Cyanidin-3- glukosid byl hlavním anthokyaninového ve fialové pšenice. Delfinidin-3-glukosid je hlavním jeden z modré pšenice. Anthokyany se považují za fyziologicky aktivních látek, jejichž význam v podpoře zdraví a omezení riziko chronických onemocnění byl dokumentován. To znamená, že přítomnost pšenice antokyanů v pigmentovaných pšenice, mohou být zdroj bioaktivních látek pro použití v potravinářském průmyslu (en)
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| Title
| - It provides Colors are anthocyanins in samples of wheat grain during maturation (en)
- Stanovni antokyanu ve vzorcich barevne psenice v prubehu zrani zrna
- Stanovni antokyanu ve vzorcich barevne psenice v prubehu zrani zrna (cs)
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| skos:prefLabel
| - It provides Colors are anthocyanins in samples of wheat grain during maturation (en)
- Stanovni antokyanu ve vzorcich barevne psenice v prubehu zrani zrna
- Stanovni antokyanu ve vzorcich barevne psenice v prubehu zrani zrna (cs)
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| skos:notation
| - RIV/62156489:43210/11:00179423!RIV12-MSM-43210___
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/62156489:43210/11:00179423
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - grain; anthocyanins; wheat (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...v/mistoKonaniAkce
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| http://linked.open...i/riv/mistoVydani
| |
| http://linked.open...i/riv/nazevZdroje
| - 18. Nove poznatky z genetiky a slachtenia polnohospodarskych rastlin
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| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...iv/tvurceVysledku
| - Húska, Dalibor
- Kizek, René
- Zítka, Ondřej
- Klejdus, Bořivoj
- Chabinová, Jana
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| http://linked.open...vavai/riv/typAkce
| |
| http://linked.open.../riv/zahajeniAkce
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| number of pages
| |
| http://purl.org/ne...btex#hasPublisher
| - Centrum vyskumu rastlinnej vyroby Piestany
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| https://schema.org/isbn
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| http://localhost/t...ganizacniJednotka
| |
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