About: Possibility of Bean yellow mosaic virus detection in gladiolus plants by different methods

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Serological and molecular methods were compared for the detection of Bean yellow mosaic virus (BYMV) in gladiolus plants. Plants showing mosaic symptoms were tested for the presence of BYMV using either DAS-ELISA, one step RT-PCR, real time (rt)-RT-PCR or IC-rt-RT-PCR. DAS-ELISA and one step RT-PCR were able to detect the virus in leaf samples, but not in corms or cormlets, while rt-RT-PCR and IC-rt-RT-PCR detected the virus in both leaves and corms or cormlets. Thus rt-RT-PCR and IC-rt-RT-PCR are proposed for the reliable diagnosis of BYMV in different tissues of gladiolus plants, particularly when virus-free stocks are required Serological and molecular methods were compared for the detection of Bean yellow mosaic virus (BYMV) in gladiolus plants. Plants showing mosaic symptoms were tested for the presence of BYMV using either DAS-ELISA, one step RT-PCR, real time (rt)-RT-PCR or IC-rt-RT-PCR. DAS-ELISA and one step RT-PCR were able to detect the virus in leaf samples, but not in corms or cormlets, while rt-RT-PCR and IC-rt-RT-PCR detected the virus in both leaves and corms or cormlets. Thus rt-RT-PCR and IC-rt-RT-PCR are proposed for the reliable diagnosis of BYMV in different tissues of gladiolus plants, particularly when virus-free stocks are required (en)
Title	Possibility of Bean yellow mosaic virus detection in gladiolus plants by different methods Possibility of Bean yellow mosaic virus detection in gladiolus plants by different methods (en)
skos:prefLabel	Possibility of Bean yellow mosaic virus detection in gladiolus plants by different methods Possibility of Bean yellow mosaic virus detection in gladiolus plants by different methods (en)
skos:notation	RIV/62156489:43210/11:00170924!RIV12-MSM-43210___
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(MSM6215648905)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Pokorný, Radovan Holková, Ludmila Duraisamy, Ganesh Selvaraj
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Mendelova univerzita v Brně / Agronomická fakulta
http://linked.open...dnocenehoVysledku	221796
http://linked.open...ai/riv/idVysledku	RIV/62156489:43210/11:00170924
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Gladiolus; bean yellow mosaic virus; RT_PCR; ELISA (en)
http://linked.open.../riv/klicoveSlovo	ELISA Gladiolus RT_PCR bean yellow mosaic virus
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[5B0831090118]
http://linked.open...i/riv/nazevZdroje	Journal of Plant Diseases and Protection
http://linked.open...in/vavai/riv/obor	GF
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	118
http://linked.open...iv/tvurceVysledku	Pokorný, Radovan Duraisamy, Ganesh Selvaraj Holková, Ludmila
http://linked.open...ain/vavai/riv/wos	289813500001
http://linked.open...n/vavai/riv/zamer	Biological and technological aspects of sustainability of controlled ecosystems and their adaptability to climate change
issn	1861-3829
number of pages	5 (xsd:int)
http://localhost/t...ganizacniJednotka	43210

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