About: The evaluation of reference genes for gene expression studies in pig muscle tissue with real-time PCR

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About: The evaluation of reference genes for gene expression studies in pig muscle tissue with real-time PCR Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The selection of reference genes is essential for gene expression studies when using real-time quantitative PCR. Reference gene selection should be performed for each individual experiment because the gene expression level may change in different experimental conditions. In this study, the stability of mRNA expression was determined for seven genes: HPRT1, RPS18, NACA, TBP, TAF4B, RPL32 and OAZ1. The stability of these reference genes was investigated in the skeletal muscle tissue of pig foetuses, piglets and adult pigs using real-time quantitative PCR and SYBR green chemistry. The expression stability of used reference genes was calculated using geNorm application. Different gene expression profiles between the age categories of pigs were found. RPS18 has been identified as the gene with the most stable expression in muscle tissue of all pig age categories. HPRT1 and RPL32 were found to have the highest stability in piglets and adult pigs, and in foetuses and adults pigs, respectively. The newly used reference gene, TAF4B, reached the highest expression stability in piglets. The selection of reference genes is essential for gene expression studies when using real-time quantitative PCR. Reference gene selection should be performed for each individual experiment because the gene expression level may change in different experimental conditions. In this study, the stability of mRNA expression was determined for seven genes: HPRT1, RPS18, NACA, TBP, TAF4B, RPL32 and OAZ1. The stability of these reference genes was investigated in the skeletal muscle tissue of pig foetuses, piglets and adult pigs using real-time quantitative PCR and SYBR green chemistry. The expression stability of used reference genes was calculated using geNorm application. Different gene expression profiles between the age categories of pigs were found. RPS18 has been identified as the gene with the most stable expression in muscle tissue of all pig age categories. HPRT1 and RPL32 were found to have the highest stability in piglets and adult pigs, and in foetuses and adults pigs, respectively. The newly used reference gene, TAF4B, reached the highest expression stability in piglets. (en)
Title	The evaluation of reference genes for gene expression studies in pig muscle tissue with real-time PCR The evaluation of reference genes for gene expression studies in pig muscle tissue with real-time PCR (en)
skos:prefLabel	The evaluation of reference genes for gene expression studies in pig muscle tissue with real-time PCR The evaluation of reference genes for gene expression studies in pig muscle tissue with real-time PCR (en)
skos:notation	RIV/62156489:43210/11:00166608!RIV12-GA0-43210___
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(GA523/09/0844)
http://linked.open...iv/cisloPeriodika	5
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Knoll, Aleš Nesvadbová, Michaela
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Mendelova univerzita v Brně / Agronomická fakulta
http://linked.open...dnocenehoVysledku	198346
http://linked.open...ai/riv/idVysledku	RIV/62156489:43210/11:00166608
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	reference gene; pig (en)
http://linked.open.../riv/klicoveSlovo	reference gene pig
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[CF11B43FD9BE]
http://linked.open...i/riv/nazevZdroje	Czech Journal of Animal Science
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	2 (xsd:int)
http://linked.open...vavai/riv/projekt	Towards understanding the biology of porcine muscle growth: Comparative gene expression analysis between skeletal muscles of Large White and Wild Boar
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	56
http://linked.open...iv/tvurceVysledku	Knoll, Aleš Nesvadbová, Michaela
issn	1212-1819
number of pages	4 (xsd:int)
http://localhost/t...ganizacniJednotka	43210

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