About: Improving of an in vitro method to estimate degradation rate of small particles of starch

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	https://mnet.mendelu.cz/mendelnet2010/mendelnet_2010_fulltext.pdf
Description	The aim of this experiment was to improve the washing process during in vitro estimation of degradation rate of starch particles. The knowledge of degradation rate of starch particles in the rumen is needed to enhance micro-bacterial protein synthesis and energy supply for dairy cows. The washout of very small particles of starch (M-fraction) in the rumen prevents determination of its degradation rate in situ. However, it is possible to measure in vitro degradation rate of both a non-washable, potentially degradable fraction (D) and the M fraction. Centrifugation appeared to be a faster, easier and more reliable method to collect the M fraction of various cereal grains compared to filtration. Medium test bags of 50 cm by 33.3 cm were found to be the most effective for collection of M and D fractions in maize, peas and barley. Results obtained with in vitro incubation with amyloglucosidase did not present satisfactory starch degradation curves. Further research will be conducted to improve this technique, or to replace it with an alternative method, such as in vitro incubation in rumen fluid. The aim of this experiment was to improve the washing process during in vitro estimation of degradation rate of starch particles. The knowledge of degradation rate of starch particles in the rumen is needed to enhance micro-bacterial protein synthesis and energy supply for dairy cows. The washout of very small particles of starch (M-fraction) in the rumen prevents determination of its degradation rate in situ. However, it is possible to measure in vitro degradation rate of both a non-washable, potentially degradable fraction (D) and the M fraction. Centrifugation appeared to be a faster, easier and more reliable method to collect the M fraction of various cereal grains compared to filtration. Medium test bags of 50 cm by 33.3 cm were found to be the most effective for collection of M and D fractions in maize, peas and barley. Results obtained with in vitro incubation with amyloglucosidase did not present satisfactory starch degradation curves. Further research will be conducted to improve this technique, or to replace it with an alternative method, such as in vitro incubation in rumen fluid. (en)
Title	Improving of an in vitro method to estimate degradation rate of small particles of starch Improving of an in vitro method to estimate degradation rate of small particles of starch (en)
skos:prefLabel	Improving of an in vitro method to estimate degradation rate of small particles of starch Improving of an in vitro method to estimate degradation rate of small particles of starch (en)
skos:notation	RIV/62156489:43210/10:00234851!RIV15-MSM-43210___
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(MSM6215648905)
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Doležal, Petr Čermáková, Jana
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Mendelova univerzita v Brně / Agronomická fakulta
http://linked.open...dnocenehoVysledku	263214
http://linked.open...ai/riv/idVysledku	RIV/62156489:43210/10:00234851
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	rumen; degradation; starch; in vitro (en)
http://linked.open.../riv/klicoveSlovo	degradation rumen starch in vitro
http://linked.open...ontrolniKodProRIV	[CD21C40C303E]
http://linked.open...v/mistoKonaniAkce	Brno
http://linked.open...i/riv/mistoVydani	Brno
http://linked.open...i/riv/nazevZdroje	MendelNet 2010 - Proceedings of International Ph.D. Students Conference
http://linked.open...in/vavai/riv/obor	GH
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...UplatneniVysledku	2010
http://linked.open...iv/tvurceVysledku	Doležal, Petr Kudrna, Václav Čermáková, Jana
http://linked.open...vavai/riv/typAkce	EUR - Evropská
http://linked.open...ain/vavai/riv/wos	341786800025
http://linked.open.../riv/zahajeniAkce	2010-11-24 (xsd:date)
http://linked.open...n/vavai/riv/zamer	Biological and technological aspects of sustainability of controlled ecosystems and their adaptability to climate change
number of pages	11 (xsd:int)
http://purl.org/ne...btex#hasPublisher	Mendelova zemědělská a lesnická univerzita v Brně
https://schema.org/isbn	978-80-7375-453-2
http://localhost/t...ganizacniJednotka	43210

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