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| Description
| - Development and aplication of uElution plate for the extraction of phenolic compounds from sea algae and their analysis using RRLC/MS/MS is described. The extraction and identification of phenolic compounds is presented from five different sea algae samples, two brown algae (Cystoseira abies + Cystoseira abies dried with nitrogen , Undaria pinnatifida) and two red algae (Sargassum muticum, Chondrus crispus) via solid phase extraction (SPE) using Oasis uElution plate. Selected groups of benzoic acid derivatives (p-hydroxybenzoic, protocatechuic, gallic, vanillic and syringic acid), hydroxybenzaldehydes (4-hydroxybenzaldehyde and 3,4- dihydroxybenzaldehyde) and cinnamic acid derivatives (o-coumaric, p-coumaric, caffeic, ferulic, sinapic and chlorogenic acid) were investigated. Recoveries in range 96--100% were obtained, with LOQs 0.01--2.1 pg/inj and LODs 0.03--7.1 pg/inj, i.e. in the range of low um. The uSPE enabled to avoid the evaporation step and preconcentrate the analytes directly. The applied method allowed a simultaneous determination of phenols in less than 5 minutes. Thus, the analysis of different plant species containing trace amounts of polar phenols became possible. Phenolic compounds contained in algae were extracted using Oasis uElution plate for its increased sensitivity and pre-concentration effect -- extract volume is only 500 ul. Robotic mechanism of the HPLC instrument is able to use the uElution plate trapping part directly and take samples from it.
- Development and aplication of uElution plate for the extraction of phenolic compounds from sea algae and their analysis using RRLC/MS/MS is described. The extraction and identification of phenolic compounds is presented from five different sea algae samples, two brown algae (Cystoseira abies + Cystoseira abies dried with nitrogen , Undaria pinnatifida) and two red algae (Sargassum muticum, Chondrus crispus) via solid phase extraction (SPE) using Oasis uElution plate. Selected groups of benzoic acid derivatives (p-hydroxybenzoic, protocatechuic, gallic, vanillic and syringic acid), hydroxybenzaldehydes (4-hydroxybenzaldehyde and 3,4- dihydroxybenzaldehyde) and cinnamic acid derivatives (o-coumaric, p-coumaric, caffeic, ferulic, sinapic and chlorogenic acid) were investigated. Recoveries in range 96--100% were obtained, with LOQs 0.01--2.1 pg/inj and LODs 0.03--7.1 pg/inj, i.e. in the range of low um. The uSPE enabled to avoid the evaporation step and preconcentrate the analytes directly. The applied method allowed a simultaneous determination of phenols in less than 5 minutes. Thus, the analysis of different plant species containing trace amounts of polar phenols became possible. Phenolic compounds contained in algae were extracted using Oasis uElution plate for its increased sensitivity and pre-concentration effect -- extract volume is only 500 ul. Robotic mechanism of the HPLC instrument is able to use the uElution plate trapping part directly and take samples from it. (en)
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| Title
| - THE USE OF MICROELUTION PLATE AS A PART OF HYPHENATED EXTRACTION METHOD FOLLOWED BY RRLC-MS-MS ANALYSIS FOR THE ISOLATION AND DETERMINATION OF PHENOLIC COMPOUNDS IN SEA ALGAE
- THE USE OF MICROELUTION PLATE AS A PART OF HYPHENATED EXTRACTION METHOD FOLLOWED BY RRLC-MS-MS ANALYSIS FOR THE ISOLATION AND DETERMINATION OF PHENOLIC COMPOUNDS IN SEA ALGAE (en)
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| skos:prefLabel
| - THE USE OF MICROELUTION PLATE AS A PART OF HYPHENATED EXTRACTION METHOD FOLLOWED BY RRLC-MS-MS ANALYSIS FOR THE ISOLATION AND DETERMINATION OF PHENOLIC COMPOUNDS IN SEA ALGAE
- THE USE OF MICROELUTION PLATE AS A PART OF HYPHENATED EXTRACTION METHOD FOLLOWED BY RRLC-MS-MS ANALYSIS FOR THE ISOLATION AND DETERMINATION OF PHENOLIC COMPOUNDS IN SEA ALGAE (en)
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| skos:notation
| - RIV/62156489:43210/10:00234796!RIV15-GA0-43210___
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/62156489:43210/10:00234796
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - sea algae; hyphenated techniques; phenolic compounds (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...v/mistoKonaniAkce
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| http://linked.open...i/riv/mistoVydani
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| http://linked.open...i/riv/nazevZdroje
| - MendelNet 2010 - Proceedings of International Ph.D. Students Conference
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...iv/tvurceVysledku
| - Vlček, Jiří
- Klejdus, Bořivoj
- Lojková, Lea
- Plaza, Merichel
- Bartáková, Marie
- Dvořáčková, Eva
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| http://linked.open...vavai/riv/typAkce
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| http://linked.open...ain/vavai/riv/wos
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| http://linked.open.../riv/zahajeniAkce
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| number of pages
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| http://purl.org/ne...btex#hasPublisher
| - Mendelova zemědělská a lesnická univerzita v Brně
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| https://schema.org/isbn
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| http://localhost/t...ganizacniJednotka
| |
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