| Attributes | Values |
|---|
| rdf:type
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| Description
| - Background: Metallothioneins (MTs) are heavy-metal binding cysteine-rich proteins. MTs are thought to play a role in tumour disease. They can be used for studying of course, prognosis and metastating of a tumour. The enhanced MTs expression leads to the formation of cytostatics resistance in tumour cells. One the current accepted opinion is that these mechanisms are closely connected to ability of MT to bind heavy metal ions and form aggregates. The aim of this work was to study the changes in aggregation of MT under various concentrations of zinc and different redox conditions in human prostatic cell lines. Material and Methods: Reduced apo-MT was oxidized with 0.75 and 3.5 % H2O2, 0.5 and 1 mMK2Cr2O7, and 0.5 and 1 mM KMnO4 for 2 hours at 37C. To the reduced and oxidized apo-MT ZnCl2 was added. Reaction was measured spectrometrically for 1 hour at 37 C. MT oxidation and interaction with zinc was evaluated by measuring of spectra within the range from 200 to 300 nm. Human cancer prostatic cell line (PC-3) and control cell line (PNT1A) were used. Results: In in vitro experiments we observed Zn binding and aggregation of both (reduced and oxidized) forms of MT. The oxidized observed a peak with maximum at 250 nm. The height of signals of both forms increased with time of the interaction and ZnCl2 concentration. Comparing reduced and oxidized MT, the oxidized molecules had higher binding capacity. The increased formation of MT aggregates in molecular weight higher than 75 kDa was observed in dependence on ZnCl2 concentration and degree of oxidation as a proportion of the most intense signal at 25 kDa measured by Experion. In cancer cell lines increased viability was observed compared to controls and the same MT aggregates were observed as at standards in dependence on ZnCl2 concentration. Conclusion: It can be concluded, that zinc supports MT expression and growth in human prostatic cell lines and that MT forms aggregates in dependence on redox conditions and zinc
- Background: Metallothioneins (MTs) are heavy-metal binding cysteine-rich proteins. MTs are thought to play a role in tumour disease. They can be used for studying of course, prognosis and metastating of a tumour. The enhanced MTs expression leads to the formation of cytostatics resistance in tumour cells. One the current accepted opinion is that these mechanisms are closely connected to ability of MT to bind heavy metal ions and form aggregates. The aim of this work was to study the changes in aggregation of MT under various concentrations of zinc and different redox conditions in human prostatic cell lines. Material and Methods: Reduced apo-MT was oxidized with 0.75 and 3.5 % H2O2, 0.5 and 1 mMK2Cr2O7, and 0.5 and 1 mM KMnO4 for 2 hours at 37C. To the reduced and oxidized apo-MT ZnCl2 was added. Reaction was measured spectrometrically for 1 hour at 37 C. MT oxidation and interaction with zinc was evaluated by measuring of spectra within the range from 200 to 300 nm. Human cancer prostatic cell line (PC-3) and control cell line (PNT1A) were used. Results: In in vitro experiments we observed Zn binding and aggregation of both (reduced and oxidized) forms of MT. The oxidized observed a peak with maximum at 250 nm. The height of signals of both forms increased with time of the interaction and ZnCl2 concentration. Comparing reduced and oxidized MT, the oxidized molecules had higher binding capacity. The increased formation of MT aggregates in molecular weight higher than 75 kDa was observed in dependence on ZnCl2 concentration and degree of oxidation as a proportion of the most intense signal at 25 kDa measured by Experion. In cancer cell lines increased viability was observed compared to controls and the same MT aggregates were observed as at standards in dependence on ZnCl2 concentration. Conclusion: It can be concluded, that zinc supports MT expression and growth in human prostatic cell lines and that MT forms aggregates in dependence on redox conditions and zinc (en)
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| Title
| - Study of zinc-dependent aggregation of metallothionein from human prostatic cancer cell lines
- Study of zinc-dependent aggregation of metallothionein from human prostatic cancer cell lines (en)
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| skos:prefLabel
| - Study of zinc-dependent aggregation of metallothionein from human prostatic cancer cell lines
- Study of zinc-dependent aggregation of metallothionein from human prostatic cancer cell lines (en)
|
| skos:notation
| - RIV/62156489:43210/10:00184955!RIV12-AV0-43210___
|
| http://linked.open...avai/riv/aktivita
| |
| http://linked.open...avai/riv/aktivity
| - P(GP301/09/P436), P(IAA401990701), P(KAN208130801)
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| http://linked.open...iv/cisloPeriodika
| |
| http://linked.open...vai/riv/dodaniDat
| |
| http://linked.open...aciTvurceVysledku
| |
| http://linked.open.../riv/druhVysledku
| |
| http://linked.open...iv/duvernostUdaju
| |
| http://linked.open...titaPredkladatele
| |
| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/62156489:43210/10:00184955
|
| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - prostatic; metallothionein; aggregation (en)
|
| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...odStatuVydavatele
| - GB - Spojené království Velké Británie a Severního Irska
|
| http://linked.open...ontrolniKodProRIV
| |
| http://linked.open...i/riv/nazevZdroje
| - European Journal of Cancer
|
| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...vavai/riv/projekt
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...v/svazekPeriodika
| |
| http://linked.open...iv/tvurceVysledku
| - Eckschlager, Tomáš
- Hubálek, Jaromír
- Kizek, René
- Křížková, Soňa
- Masařík, Michal
- Průša, Richard
- Kukačka, Jiří
|
| issn
| |
| number of pages
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| http://localhost/t...ganizacniJednotka
| |
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