About: HPLC with electrochemical detection for studying of synthesis of phytochelatins in cadmium treated flax

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About: HPLC with electrochemical detection for studying of synthesis of phytochelatins in cadmium treated flax Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	If there would be free heavy metals occurring in the organism without control it initiates creation of number of dangerous species (ROS). These species are disturbing of homeostasis of organism by subsequent oxidation of essential cellular structures as nucleic acids. Animal and plant organism has both one efficient defending system which includes synthesis of glutathione (GSH). But plants are limited in case of escape from metal contaminated area. Therefore plant cell has mechanism includes synthesis of phytochelatins (PC) from GSH by phytochelatin synthase. PC's are able to immobilize heavy metal ion via number of thiol groups. We treated the plants of flax (Linum usativum) by various concentration of Cd(II) ions (50, 250, and 500mM) in our study. Subsequently we used our developed method for determination of oxidation stress as GSH/GSSG ratio and levels of PC-2,3,4 and 5. We observed that most synthesised PC under the highest concentration of Cd(II) treatment was PC-2,4 and 5. If there would be free heavy metals occurring in the organism without control it initiates creation of number of dangerous species (ROS). These species are disturbing of homeostasis of organism by subsequent oxidation of essential cellular structures as nucleic acids. Animal and plant organism has both one efficient defending system which includes synthesis of glutathione (GSH). But plants are limited in case of escape from metal contaminated area. Therefore plant cell has mechanism includes synthesis of phytochelatins (PC) from GSH by phytochelatin synthase. PC's are able to immobilize heavy metal ion via number of thiol groups. We treated the plants of flax (Linum usativum) by various concentration of Cd(II) ions (50, 250, and 500mM) in our study. Subsequently we used our developed method for determination of oxidation stress as GSH/GSSG ratio and levels of PC-2,3,4 and 5. We observed that most synthesised PC under the highest concentration of Cd(II) treatment was PC-2,4 and 5. (en)
Title	HPLC with electrochemical detection for studying of synthesis of phytochelatins in cadmium treated flax HPLC with electrochemical detection for studying of synthesis of phytochelatins in cadmium treated flax (en)
skos:prefLabel	HPLC with electrochemical detection for studying of synthesis of phytochelatins in cadmium treated flax HPLC with electrochemical detection for studying of synthesis of phytochelatins in cadmium treated flax (en)
skos:notation	RIV/62156489:43210/10:00184621!RIV12-MSM-43210___
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(1M06030), P(GA522/09/0239)
http://linked.open...iv/cisloPeriodika	5
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Cernei, Natalia Vladimirovna Adam, Vojtěch Horna, Aleš Kizek, René Zítka, Ondřej
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Mendelova univerzita v Brně / Agronomická fakulta
http://linked.open...dnocenehoVysledku	262219
http://linked.open...ai/riv/idVysledku	RIV/62156489:43210/10:00184621
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	cellular structure cellular structure; synthesis; electrochemical detection (en)
http://linked.open.../riv/klicoveSlovo	electrochemical detection synthesis cellular structure cellular structure
http://linked.open...odStatuVydavatele	IN - Indická republika
http://linked.open...ontrolniKodProRIV	[B0CF9A940768]
http://linked.open...i/riv/nazevZdroje	Journal of Biochemical Technology
http://linked.open...in/vavai/riv/obor	CB
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Functional genomics and proteomics for crop improvement Cellular and organ responses of plants to the polycyclic aromatic hydrocarbon
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	2
http://linked.open...iv/tvurceVysledku	Adam, Vojtěch Cernei, Natalia Vladimirovna Horna, Aleš Kizek, René Zítka, Ondřej Najmanova, Jitka
issn	0974-2328
number of pages	2 (xsd:int)
http://localhost/t...ganizacniJednotka	43210

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