About: Easy to use and rapid isolation and detection of a viral nucleic acid by using of paramagnetic microparticles and carbon nanotubes-based screen-printed electrodes

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About: Easy to use and rapid isolation and detection of a viral nucleic acid by using of paramagnetic microparticles and carbon nanotubes-based screen-printed electrodes Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	A method that is easy to use, rapid, with a low cost of detecting viral nucleic acid in a biological sample represents the essential tool in targeted therapy. In this study, we report the use of paramagnetic microparticles covered by streptavidin and modified by an oligonucleotide probe with a specific viral sequence labeled by biotin to detect human immunodeficiency virus (HIV) and influenza virus subtype H5N1. The viral nucleic acids were primarily detected by adsorptive transfer stripping technique coupled with square wave voltammetry using carbon paste, hanging mercury drop or carbon nanotubes-based screen-printed working electrodes. Detection limits were estimated for both sequences down to picograms per 3 ul. To isolate the viral sequences, paramagnetic microparticles covered with biotin-labeled oligonucleotides were used. We calculated the yield of isolation for H5N1 and/or HIV sequences, which was defined as %22isolated concentration of viral nucleic acid sequence%22/%22given viral nucleic acid sequ A method that is easy to use, rapid, with a low cost of detecting viral nucleic acid in a biological sample represents the essential tool in targeted therapy. In this study, we report the use of paramagnetic microparticles covered by streptavidin and modified by an oligonucleotide probe with a specific viral sequence labeled by biotin to detect human immunodeficiency virus (HIV) and influenza virus subtype H5N1. The viral nucleic acids were primarily detected by adsorptive transfer stripping technique coupled with square wave voltammetry using carbon paste, hanging mercury drop or carbon nanotubes-based screen-printed working electrodes. Detection limits were estimated for both sequences down to picograms per 3 ul. To isolate the viral sequences, paramagnetic microparticles covered with biotin-labeled oligonucleotides were used. We calculated the yield of isolation for H5N1 and/or HIV sequences, which was defined as %22isolated concentration of viral nucleic acid sequence%22/%22given viral nucleic acid sequ (en)
Title	Easy to use and rapid isolation and detection of a viral nucleic acid by using of paramagnetic microparticles and carbon nanotubes-based screen-printed electrodes Easy to use and rapid isolation and detection of a viral nucleic acid by using of paramagnetic microparticles and carbon nanotubes-based screen-printed electrodes (en)
skos:prefLabel	Easy to use and rapid isolation and detection of a viral nucleic acid by using of paramagnetic microparticles and carbon nanotubes-based screen-printed electrodes Easy to use and rapid isolation and detection of a viral nucleic acid by using of paramagnetic microparticles and carbon nanotubes-based screen-printed electrodes (en)
skos:notation	RIV/62156489:43210/10:00166433!RIV11-GA0-43210___
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(GA102/08/1546), P(KAN208130801)
http://linked.open...iv/cisloPeriodika	3
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Húska, Dalibor Adam, Vojtěch Hubálek, Jaromír Kizek, René
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Mendelova univerzita v Brně / Agronomická fakulta
http://linked.open...dnocenehoVysledku	255657
http://linked.open...ai/riv/idVysledku	RIV/62156489:43210/10:00166433
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	voltammetry; microparticles; nucleic acid (en)
http://linked.open.../riv/klicoveSlovo	microparticles nucleic acid voltammetry
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[BDE36F22B106]
http://linked.open...i/riv/nazevZdroje	Microfluidics and Nanofluidics
http://linked.open...in/vavai/riv/obor	CG
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	New design and exploiting nanobiosensors and nanosensors to target medicine (NANOSEMED) Miniaturized intelligent systems and nanostructured elektrodes for chemical, biological, and pharmaceutical applications (NANIMEL)
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	8
http://linked.open...iv/tvurceVysledku	Adam, Vojtěch Hubálek, Jaromír Húska, Dalibor Kizek, René
issn	1613-4982
number of pages	11 (xsd:int)
http://localhost/t...ganizacniJednotka	43210

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