About: Taq man allelic discrimination assay for A1 and A2 alleles of the bovine CSN2 gene

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About: Taq man allelic discrimination assay for A1 and A2 alleles of the bovine CSN2 gene Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Alleles A1 and A2 of the Bos taurus CSN2 gene are the most frequent in a number of dairy cattle breeds. In this study, a new allelic discrimination assay using TaqMan fluorogenic probes was developed to detect single nucleotide substitution characterizing the A1/A2 alleles of the CSN2 gene. The method was validated using DNA samples of known genotypes with different concentrations and the results were compared with those for the commonly used problematic ACRS-PCR. We found the TaqMan method to be more effective, 100% reliable and hundred times more sensitive for testing the CSN2 genetic marker in cattle than the ACRS-PCR. As it enabled a rapid analysis of a large number of DNA samples in uniform format without previous DNA quality assessment and without the requirement for post-amplification manipulations, it presents an effective tool for the analysis of large-scale sample sets. The method was applied for testing on a sample of 120 Czech Holstein dairy cows. The observed relative genotype and allel Alleles A1 and A2 of the Bos taurus CSN2 gene are the most frequent in a number of dairy cattle breeds. In this study, a new allelic discrimination assay using TaqMan fluorogenic probes was developed to detect single nucleotide substitution characterizing the A1/A2 alleles of the CSN2 gene. The method was validated using DNA samples of known genotypes with different concentrations and the results were compared with those for the commonly used problematic ACRS-PCR. We found the TaqMan method to be more effective, 100% reliable and hundred times more sensitive for testing the CSN2 genetic marker in cattle than the ACRS-PCR. As it enabled a rapid analysis of a large number of DNA samples in uniform format without previous DNA quality assessment and without the requirement for post-amplification manipulations, it presents an effective tool for the analysis of large-scale sample sets. The method was applied for testing on a sample of 120 Czech Holstein dairy cows. The observed relative genotype and allel (en)
Title	Taq man allelic discrimination assay for A1 and A2 alleles of the bovine CSN2 gene Taq man allelic discrimination assay for A1 and A2 alleles of the bovine CSN2 gene (en)
skos:prefLabel	Taq man allelic discrimination assay for A1 and A2 alleles of the bovine CSN2 gene Taq man allelic discrimination assay for A1 and A2 alleles of the bovine CSN2 gene (en)
skos:notation	RIV/62156489:43210/10:00160403!RIV11-MZE-43210___
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(1G58073)
http://linked.open...iv/cisloPeriodika	8
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Dvořák, Josef
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Mendelova univerzita v Brně / Agronomická fakulta
http://linked.open...dnocenehoVysledku	291817
http://linked.open...ai/riv/idVysledku	RIV/62156489:43210/10:00160403
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	CSN2; allelic discrimination; beef cattle; a2 allele (en)
http://linked.open.../riv/klicoveSlovo	CSN2 a2 allele beef cattle allelic discrimination
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[32043F6B62BD]
http://linked.open...i/riv/nazevZdroje	Czech journal of animal science
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	2 (xsd:int)
http://linked.open...vavai/riv/projekt	The research and pilot validation of genomic methods applicable to the selection of quality and commercial utilisation of farm animals and their products.
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	55
http://linked.open...iv/tvurceVysledku	Dvořák, Josef Manga, Ivan
http://linked.open...ain/vavai/riv/wos	281109000001
issn	1212-1819
number of pages	6 (xsd:int)
http://localhost/t...ganizacniJednotka	43210

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