About: Comparative analysis of ELISA, one step RT-PCR and IC-RT-PCR for the detection of Bean yellow mosaic virus in gladiolus     Goto   Sponge   NotDistinct   Permalink

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Description
  • To overcome the problematic detection Bean yellow mosaic virus (BYMV) in gladiolus corm and cormlets, we tested suitability of different diagnostic methods such as ELISA, one step RT-PCR and IC-PCR. Among these methods, DAS-ELISA and one step RT-PCR was able to detect in leaves and failed to detect in corms or cormlets whereas IC-RT-PCR method was very sensitive and able to detect virus in both gladiolus leaves and corm or cormlets.
  • To overcome the problematic detection Bean yellow mosaic virus (BYMV) in gladiolus corm and cormlets, we tested suitability of different diagnostic methods such as ELISA, one step RT-PCR and IC-PCR. Among these methods, DAS-ELISA and one step RT-PCR was able to detect in leaves and failed to detect in corms or cormlets whereas IC-RT-PCR method was very sensitive and able to detect virus in both gladiolus leaves and corm or cormlets. (en)
Title
  • Comparative analysis of ELISA, one step RT-PCR and IC-RT-PCR for the detection of Bean yellow mosaic virus in gladiolus
  • Comparative analysis of ELISA, one step RT-PCR and IC-RT-PCR for the detection of Bean yellow mosaic virus in gladiolus (en)
skos:prefLabel
  • Comparative analysis of ELISA, one step RT-PCR and IC-RT-PCR for the detection of Bean yellow mosaic virus in gladiolus
  • Comparative analysis of ELISA, one step RT-PCR and IC-RT-PCR for the detection of Bean yellow mosaic virus in gladiolus (en)
skos:notation
  • RIV/62156489:43210/09:00161014!RIV11-MSM-43210___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • Z(MSM6215648905)
http://linked.open...iv/cisloPeriodika
  • 3
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 307635
http://linked.open...ai/riv/idVysledku
  • RIV/62156489:43210/09:00161014
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Gladiolus; Bean yellow mosaic virus; ELISA; RT-PCR; IC-RT-PCR (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • BE - Belgické království
http://linked.open...ontrolniKodProRIV
  • [9B8CC9429BDF]
http://linked.open...i/riv/nazevZdroje
  • Communications in Agricultural and Applied Biological Sciences
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 74
http://linked.open...iv/tvurceVysledku
  • Pokorný, Radovan
  • Duraisamy, Ganesh Selvaraj
  • Holková, Ludmila
http://linked.open...n/vavai/riv/zamer
issn
  • 1379-1176
number of pages
http://localhost/t...ganizacniJednotka
  • 43210
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