About: Optimisation of qualitative and semi-quantitative detection of genetically modified crops by PCR

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	For qualitative and semi-quantitative detection of genetically modified maize we used two maize genotypes from the company Monsanto; i.e. a MON810-based transgenic hybrid of Bt-maize and a genetically non-modified control (isogenic line of Bt-maize). The seeds were the subject matter of a %22material transfer agreement%22 between Monsanto Europe and MUAF in Brno. For molecular analysis the genomic DNA was isolated from cultivated seedlings in the stage of one leaf (10-day-old plants grown at a temperature of 20oC and light regime of 12 h light and 12 h darkness) using the isolation kit DNeasy Plant Mini Kit. The plants were cultivated and genomic DNA was isolated in rooms intended for handling GMO's. After isolation the DNA concentration in the sample was determined fluorimetrically. We used primers and PCR programmes published by Greiner et al. (2005) and Hernandéz et al. (2005). When introducing PCR methods of detection of Bt-maize the first step was to optimise the protocol for the detection of the mai For qualitative and semi-quantitative detection of genetically modified maize we used two maize genotypes from the company Monsanto; i.e. a MON810-based transgenic hybrid of Bt-maize and a genetically non-modified control (isogenic line of Bt-maize). The seeds were the subject matter of a %22material transfer agreement%22 between Monsanto Europe and MUAF in Brno. For molecular analysis the genomic DNA was isolated from cultivated seedlings in the stage of one leaf (10-day-old plants grown at a temperature of 20oC and light regime of 12 h light and 12 h darkness) using the isolation kit DNeasy Plant Mini Kit. The plants were cultivated and genomic DNA was isolated in rooms intended for handling GMO's. After isolation the DNA concentration in the sample was determined fluorimetrically. We used primers and PCR programmes published by Greiner et al. (2005) and Hernandéz et al. (2005). When introducing PCR methods of detection of Bt-maize the first step was to optimise the protocol for the detection of the mai (en)
Title	Optimisation of qualitative and semi-quantitative detection of genetically modified crops by PCR Optimisation of qualitative and semi-quantitative detection of genetically modified crops by PCR (en)
skos:prefLabel	Optimisation of qualitative and semi-quantitative detection of genetically modified crops by PCR Optimisation of qualitative and semi-quantitative detection of genetically modified crops by PCR (en)
skos:notation	RIV/62156489:43210/09:00144430!RIV10-MSM-43210___
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(1M06030)
http://linked.open...iv/cisloPeriodika	5
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Hanáček, Pavel Vyhnánek, Tomáš
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Mendelova univerzita v Brně / Agronomická fakulta
http://linked.open...dnocenehoVysledku	331990
http://linked.open...ai/riv/idVysledku	RIV/62156489:43210/09:00144430
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	PCR; transgenic crops; qualitative detection; maize MON810; semi-quantitative detection; multiplex PCR (en)
http://linked.open.../riv/klicoveSlovo	maize MON810 qualitative detection transgenic crops PCR multiplex PCR semi-quantitative detection
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[C13639CADBA9]
http://linked.open...i/riv/nazevZdroje	Acta Universitatis agriculturae et silviculturae Mendelianae Brunensis : Acta of Mendel University of agriculture and forestry Brno = Acta Mendelovy zemědělské a lesnické univerzity v Brně
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	2 (xsd:int)
http://linked.open...vavai/riv/projekt	Functional genomics and proteomics for crop improvement
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	LVII
http://linked.open...iv/tvurceVysledku	Hanáček, Pavel Vyhnánek, Tomáš
issn	1211-8516
number of pages	6 (xsd:int)
http://localhost/t...ganizacniJednotka	43210

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