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| - The objective of the present study was to explore the polymorphism of microsatellite markers localised on chromosomes 3H and 7H in 15 genotypes of barley (Hordeum vulgare L.), spring form (2n = 2x = 14 chromosomes, genome HVHV) from the collection of genetic resources of the Agricultural Research Institute Kroměříž, Ltd. showing various degrees of susceptibility to Rhynchosporium secalis. The selection of SSR markers was based on hitherto achieved knowledge according to which the greatest amount of resistance genes against Rhynchosporium secalis is localised on chromosomes 3H and 7H of barley. We selected 33 SSR markers for the analyses; 17 were localised on chromosome 3H of barley and 16 on chromosome 7H. Out of the total 33 SSR markers, 32 were polymorphous and one marker (Bmac0282) was monomorphic. In total we detected 172 alleles ranging between 101 and 235 bp; the average number of alleles per locus was 5.21. In terms of the polymorphism of the SSR markers localised on chromosomes 3H and 7H the
- The objective of the present study was to explore the polymorphism of microsatellite markers localised on chromosomes 3H and 7H in 15 genotypes of barley (Hordeum vulgare L.), spring form (2n = 2x = 14 chromosomes, genome HVHV) from the collection of genetic resources of the Agricultural Research Institute Kroměříž, Ltd. showing various degrees of susceptibility to Rhynchosporium secalis. The selection of SSR markers was based on hitherto achieved knowledge according to which the greatest amount of resistance genes against Rhynchosporium secalis is localised on chromosomes 3H and 7H of barley. We selected 33 SSR markers for the analyses; 17 were localised on chromosome 3H of barley and 16 on chromosome 7H. Out of the total 33 SSR markers, 32 were polymorphous and one marker (Bmac0282) was monomorphic. In total we detected 172 alleles ranging between 101 and 235 bp; the average number of alleles per locus was 5.21. In terms of the polymorphism of the SSR markers localised on chromosomes 3H and 7H the (en)
- Cílem práce bylo studium polymorfismu mikrosatelitních markerů na 3H a 7H chromozomu ječmene u genotypů ječmene rezistentních a náchylných k Rhynchosporium secalis. Soubor patnácti analyzovaných genotypů ječmene setého (Hordeum vulgare L.) jarní formy pochází z kolekce genetických zdrojů Zemědělského výzkumného ústavu Kroměříž, s.r.o.: Abyssinian, Atlas, Atlas 46, Atlas 57, Bay, Cambrinus, Clipper, Jet, Kitchin, Kompakt, Korál, La Mesita, Nigrinudum Abyssinum, Psaknon a Rapid. K analýzám bylo vybráno celkem 33 SSR markerů, z nichž 17 bylo lokalizováno na 3H chromozomu ječmene a 16 SSR markerů bylo lokalizováno na 7H chromozomu ječmene. Mikrosatelitní markery byly vybrány z databáze GrainGenes a z literatury, kde je uvedena sekvence primerů, motiv a lokalizace. Celková genomická DNA byla izolována pomocí izolačního kitu DNeasy Plant Mini Kit od firmy Qiagen (GE) z listů rostlin ve stáří 6--7 dní. Koncentrace izolované DNA byla zjišťována spektrofotometricky. Výsledné produkty PCR reakce byly elektrofor (cs)
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| Title
| - Polymorphism of microsatellite markers on chromosomes 3H and 7H in barley genotypes resistant and susceptible to Rhynchosporium secalis
- Polymorphism of microsatellite markers on chromosomes 3H and 7H in barley genotypes resistant and susceptible to Rhynchosporium secalis (en)
- Polymorfismus mikrosatelitních markerů na 3H a 7H chromozomu u genotypů ječmene rezistentních a náchylných k Rhynchosporium secalis (cs)
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| skos:prefLabel
| - Polymorphism of microsatellite markers on chromosomes 3H and 7H in barley genotypes resistant and susceptible to Rhynchosporium secalis
- Polymorphism of microsatellite markers on chromosomes 3H and 7H in barley genotypes resistant and susceptible to Rhynchosporium secalis (en)
- Polymorfismus mikrosatelitních markerů na 3H a 7H chromozomu u genotypů ječmene rezistentních a náchylných k Rhynchosporium secalis (cs)
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| skos:notation
| - RIV/62156489:43210/09:00139259!RIV09-GA0-43210___
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/62156489:43210/09:00139259
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - microsatellite markers; resistance; polymorphism; Rhynchosporium secalis (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
| - Acta Universitatis agriculturae et silviculturae Mendelianae Brunensis : Acta of Mendel University of agriculture and forestry Brno = Acta Mendelovy zemědělské a lesnické univerzity v Brně
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Bednář, Jan
- Vyhnánek, Tomáš
- Nevimová, Hana
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| issn
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| http://localhost/t...ganizacniJednotka
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