About: Long-term suspension cultures of cucumber (Cucumis sativus L.) with high embryogenic potential

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About: Long-term suspension cultures of cucumber (Cucumis sativus L.) with high embryogenic potential Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Cucumber (Cucumis sativus L.) cytokinin-independent embryogenic cell suspension cultures were derived and maintained for more than three and half years without losing the embryogenic potential. The preparation and the characteristics of the cucumber embryogenic cell suspension possess many similarities to that of carrot. The cultures were induced from hypocotyl explants of in vitro grown cucumber plants in liquid MS media containing 2,4-dichlorophenoxyacetic acid as the sole growth regulator during six weeks and they contained a heterogeneous array of several different types of single cells and cell clusters (PEMs). The established cell suspensions were subcultured in one-week intervals, while the inoculation density was optimized to 2.0 x 105 cells ml-1 using cell viability as a marker. Somatic embryos were obtained after the transfer of the proembryogenic masses to a hormone-free semisolid MS medium with a frequency of 388 plus-minus 57 somatic embryos per 1 ml of packed cell volume of the establish Cucumber (Cucumis sativus L.) cytokinin-independent embryogenic cell suspension cultures were derived and maintained for more than three and half years without losing the embryogenic potential. The preparation and the characteristics of the cucumber embryogenic cell suspension possess many similarities to that of carrot. The cultures were induced from hypocotyl explants of in vitro grown cucumber plants in liquid MS media containing 2,4-dichlorophenoxyacetic acid as the sole growth regulator during six weeks and they contained a heterogeneous array of several different types of single cells and cell clusters (PEMs). The established cell suspensions were subcultured in one-week intervals, while the inoculation density was optimized to 2.0 x 105 cells ml-1 using cell viability as a marker. Somatic embryos were obtained after the transfer of the proembryogenic masses to a hormone-free semisolid MS medium with a frequency of 388 plus-minus 57 somatic embryos per 1 ml of packed cell volume of the establish (en) Cytokinin independentní embryogenní buněčná suspenze okurky (Cucumis sativus L.) byla odvozena a udržována více než tři a půl roku bez ztráty embryogenního potenciálu. (cs)
Title	Long-term suspension cultures of cucumber (Cucumis sativus L.) with high embryogenic potential Long-term suspension cultures of cucumber (Cucumis sativus L.) with high embryogenic potential (en) Dlouhodobé suspenzní kultury okurky (Cucumis sativus L.) s vysokým embryogenním potenciálem (cs)
skos:prefLabel	Long-term suspension cultures of cucumber (Cucumis sativus L.) with high embryogenic potential Long-term suspension cultures of cucumber (Cucumis sativus L.) with high embryogenic potential (en) Dlouhodobé suspenzní kultury okurky (Cucumis sativus L.) s vysokým embryogenním potenciálem (cs)
skos:notation	RIV/62156489:43210/09:00137108!RIV09-MSM-43210___
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(MSM 432100001)
http://linked.open...iv/cisloPeriodika	00902774
http://linked.open...vai/riv/dodaniDat	2009
http://linked.open...aciTvurceVysledku	Andrýsková, Lenka Procházka, Stanislav Reinöhl, Vilém Klemš, Marek
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Mendelova univerzita v Brně / Agronomická fakulta
http://linked.open...dnocenehoVysledku	324132
http://linked.open...ai/riv/idVysledku	RIV/62156489:43210/09:00137108
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	cucumber; embryo conversion; somatic embryos; cell lines; 2,4-D (en)
http://linked.open.../riv/klicoveSlovo	somatic embryos 4-D cell lines cucumber 2 embryo conversion
http://linked.open...odStatuVydavatele	PL - Polská republika
http://linked.open...ontrolniKodProRIV	[DDE86BB6B35C]
http://linked.open...i/riv/nazevZdroje	Acta Physiologiae Plantarum
http://linked.open...in/vavai/riv/obor	EF
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	DOI 10.100
http://linked.open...iv/tvurceVysledku	Procházka, Stanislav Reinöhl, Vilém Klemš, Marek Andrýsková, Lenka
http://linked.open...n/vavai/riv/zamer	http://linked.opendata.cz/resource/domain/vavai/zamer/MSM%20432100001
issn	0137-5881
number of pages	7 (xsd:int)
http://localhost/t...ganizacniJednotka	43210

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