About: Novel detection of c131y mutation using allele specific PCR (AS

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About: Novel detection of c131y mutation using allele specific PCR (AS – PCR) Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	In the present study we deal with detecting SNP in myostatin (MSTN) gene using molecular genomic methods. MSTN is a negative regulator of muscle growth. Nine different mutations were found in that gene. These are responsible for double muscling in cattle and the others species. Mutation called G938A was detected in Gasconne cattle breed and it is causing embarrassment during birth. The aim of breeders is to suppress appearance of phenotype trait of double muscling in Gasconne sires, mainly. By mentioned reasons, two different methods were designed. First was based on allele specific PCR. This method obtained positive results with comparing of results from sequencing fragment. Novel method was verified using another one. The primers were designed using on line software Primer 3 (v 0.4.0). The samples were analysed in ABI PRISM 310 machine with using DNA Sequencing Analysis Software (ver. 5.1). In the present study we deal with detecting SNP in myostatin (MSTN) gene using molecular genomic methods. MSTN is a negative regulator of muscle growth. Nine different mutations were found in that gene. These are responsible for double muscling in cattle and the others species. Mutation called G938A was detected in Gasconne cattle breed and it is causing embarrassment during birth. The aim of breeders is to suppress appearance of phenotype trait of double muscling in Gasconne sires, mainly. By mentioned reasons, two different methods were designed. First was based on allele specific PCR. This method obtained positive results with comparing of results from sequencing fragment. Novel method was verified using another one. The primers were designed using on line software Primer 3 (v 0.4.0). The samples were analysed in ABI PRISM 310 machine with using DNA Sequencing Analysis Software (ver. 5.1). (en) Pomocí AS-PCR byla detekována mutace C313Y u plemene gasconne. Mutace je odpovědná za dvojite osvaleni u tohoto plemene. Důvěryhodnost detekce pomoci nové metody byla ověřena sekvenovanim daného úseku. (cs)
Title	Novel detection of c131y mutation using allele specific PCR (AS – PCR) Nový způsob detekce mutace C313Y pomocí alelle specific PCR (AS-PCR) (cs) Novel detection of c131y mutation using allele specific PCR (AS – PCR) (en)
skos:prefLabel	Novel detection of c131y mutation using allele specific PCR (AS – PCR) Nový způsob detekce mutace C313Y pomocí alelle specific PCR (AS-PCR) (cs) Novel detection of c131y mutation using allele specific PCR (AS – PCR) (en)
skos:notation	RIV/62156489:43210/08:9P000445!RIV09-MZE-43210___
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(1G58073), P(2B08037)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2009
http://linked.open...aciTvurceVysledku	Stehlík, Libor
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Mendelova univerzita v Brně / Agronomická fakulta
http://linked.open...dnocenehoVysledku	383262
http://linked.open...ai/riv/idVysledku	RIV/62156489:43210/08:9P000445
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	MSTN; Gasconne; AS-PCR (en)
http://linked.open.../riv/klicoveSlovo	AS-PCR Gasconne MSTN
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[0CCE829B2109]
http://linked.open...i/riv/nazevZdroje	Journal of Agrobiology
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	1 (xsd:int)
http://linked.open...vavai/riv/projekt	The research and pilot validation of genomic methods applicable to the selection of quality and commercial utilisation of farm animals and their products. Biotechnological methods for innovations of manufacturing and consumerist quality evaluation of beef as source of an animal proteins.
http://linked.open...UplatneniVysledku	2008
http://linked.open...v/svazekPeriodika	25
http://linked.open...iv/tvurceVysledku	Stehlík, Libor
issn	1803-4403
number of pages	3 (xsd:int)
http://localhost/t...ganizacniJednotka	43210

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