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  • The aim of this work was to propose new technique for isolation and detection of total content of mRNA in human plasma. The total content of plasma mRNA varied from 0.1 to 1.2 ug/ml. We succeeded in isolating mRNA by using simple technique with MPs. Recovery of the isolation of mRNA (tested on polyA chains) was about 30 %. In comparison with common techniques used for isolation of mRNA (e.g. phenol-chloroform extraction) the recovery of the MPs approach is higher and, moreover, this technique is not so time consuming, because we were able to analyse twelve plasma samples within 120 minutes.
  • The aim of this work was to propose new technique for isolation and detection of total content of mRNA in human plasma. The total content of plasma mRNA varied from 0.1 to 1.2 ug/ml. We succeeded in isolating mRNA by using simple technique with MPs. Recovery of the isolation of mRNA (tested on polyA chains) was about 30 %. In comparison with common techniques used for isolation of mRNA (e.g. phenol-chloroform extraction) the recovery of the MPs approach is higher and, moreover, this technique is not so time consuming, because we were able to analyse twelve plasma samples within 120 minutes. (en)
  • Cíl této práce byl vyvynout novou techniku pro isolaci a detekci celkové mRNA v lidské plazmě. elkový obsah mRNA v plazmé se pohybovala v rozmezí 0.1 až 1.2 ug/ml. Výtěžnost izolační metody dosahoval kolem 30 %. V porovnání s klasickými izolačními postupy chloroform-fenol měl použitý postup vyšší výtěžnost a rovněž byl i rychlejší. (cs)
Title
  • New technique for quantitative elelectrochemical determination of total plasma mRNA
  • New technique for quantitative elelectrochemical determination of total plasma mRNA (en)
  • Nové techniky pro kvantitativní elektrochemické stanovení celkové plazmatické mRNA (cs)
skos:prefLabel
  • New technique for quantitative elelectrochemical determination of total plasma mRNA
  • New technique for quantitative elelectrochemical determination of total plasma mRNA (en)
  • Nové techniky pro kvantitativní elektrochemické stanovení celkové plazmatické mRNA (cs)
skos:notation
  • RIV/62156489:43210/08:00133057!RIV09-AV0-43210___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(KAN208130801)
http://linked.open...iv/cisloPeriodika
  • 6
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 382676
http://linked.open...ai/riv/idVysledku
  • RIV/62156489:43210/08:00133057
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • mRNA (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • US - Spojené státy americké
http://linked.open...ontrolniKodProRIV
  • [EFC03EEEE14D]
http://linked.open...i/riv/nazevZdroje
  • Clinical chemistry
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 54
http://linked.open...iv/tvurceVysledku
  • Adam, Vojtěch
  • Húska, Dalibor
  • Kizek, René
  • Průša, Richard
  • Vajtr, D.
  • Alba Mejía, Jhonny Edison
  • Kukacka, J.
issn
  • 0009-9147
number of pages
http://localhost/t...ganizacniJednotka
  • 43210
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