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  • In the present work we report on investigation of affecting of duckweed (Lemna minor) by various doses of cisplatin (0, 5, 10, 20, 40, 80 and 160 uM) for 4 days. The toxic influence of cisplatin was evaluated on the basis of growth inhibition expressed as number of leaves, growth rate, and total amount of biomass. The result value of 96hEC50, calculated from growth inhibition with comparison of growth rates, was 6.93 uM. Moreover we aimed on determination of cisplatin content using differential pulse voltammetry. The highest content of cisplatin (320 ng g-1 of fresh weight) was determined in plants treated by 80 uM at the second day of treatment. Plants protect themselves against heavy metals by means of synthesis of cysteine-rich peptides such as glutathione and phytochelatins. Thus thiol determination in the treated plants by means of Brdicka reaction followed. The marked increase in thiol concentration detected is associated with defence reaction of the plant against stress caused by cisplatin.
  • In the present work we report on investigation of affecting of duckweed (Lemna minor) by various doses of cisplatin (0, 5, 10, 20, 40, 80 and 160 uM) for 4 days. The toxic influence of cisplatin was evaluated on the basis of growth inhibition expressed as number of leaves, growth rate, and total amount of biomass. The result value of 96hEC50, calculated from growth inhibition with comparison of growth rates, was 6.93 uM. Moreover we aimed on determination of cisplatin content using differential pulse voltammetry. The highest content of cisplatin (320 ng g-1 of fresh weight) was determined in plants treated by 80 uM at the second day of treatment. Plants protect themselves against heavy metals by means of synthesis of cysteine-rich peptides such as glutathione and phytochelatins. Thus thiol determination in the treated plants by means of Brdicka reaction followed. The marked increase in thiol concentration detected is associated with defence reaction of the plant against stress caused by cisplatin. (en)
  • V této práci jsme zkoumli vliv různých dávek cis-platiny (0-160uM) ma Lemna minor po dobu 4 dnů. Toxický vliv cis-platiny byl zjištěn na základě růstové inhibice vyjádřené v podobě počtu listů a růstového poměru byiomasy. Zjištěná hodnota pro poměr tvorby biomasy EC 50 po 96 hodinách byla 6.93 uM. Mimoto jsem zjistliy obsah cysplatiny pomocí diferenční pulsní voltametrie. Nejvyssi obsah byl cis-platiny 320 ng/g čerstvé hmotnosti byl zjištěn u rostlin vystavených 80 uM ve druhém dnu experimentu. Rostlinná chrana zahrnovala i tvorbu na cystein bohatých sloučebnin. (cs)
Title
  • Affecting of aquatic vascular plant Lemna minor by cisplatin revealed by voltammetry
  • Vliv cisplatiny na vodní rostlinu Lemna minor tudovaný voltametrií (cs)
  • Affecting of aquatic vascular plant Lemna minor by cisplatin revealed by voltammetry (en)
skos:prefLabel
  • Affecting of aquatic vascular plant Lemna minor by cisplatin revealed by voltammetry
  • Vliv cisplatiny na vodní rostlinu Lemna minor tudovaný voltametrií (cs)
  • Affecting of aquatic vascular plant Lemna minor by cisplatin revealed by voltammetry (en)
skos:notation
  • RIV/62156489:43210/08:00122083!RIV09-MSM-43210___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(1M06030), P(GA522/07/0692), Z(MSM6215712402)
http://linked.open...iv/cisloPeriodika
  • 1
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 355060
http://linked.open...ai/riv/idVysledku
  • RIV/62156489:43210/08:00122083
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • platin based cytostatic (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • CH - Švýcarská konfederace
http://linked.open...ontrolniKodProRIV
  • [06C202838C87]
http://linked.open...i/riv/nazevZdroje
  • Bioelectrochemistry
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 72
http://linked.open...iv/tvurceVysledku
  • Adam, Vojtěch
  • Beklová, Miroslava
  • Havel, Ladislav
  • Húska, Dalibor
  • Kizek, René
  • Pikula, Jiří
  • Zehnálek, Josef
  • Šupálková, Veronika
  • Baloun, Jiří
  • Rauscherová, L.
  • Singer, Christoph
  • Sures, Bernd
http://linked.open...n/vavai/riv/zamer
issn
  • 1567-5394
number of pages
http://localhost/t...ganizacniJednotka
  • 43210
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