About: Transformation of Pea to Induce Resistance to Insect Pests and Fungal Pathogens - First Partial Results     Goto   Sponge   NotDistinct   Permalink

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Description
  • Několik českých kultivarů hrachu bylo transformováno geny ovlivňujícími odolnost vůči škůdcům a patogenům. Tři konstrukty obsahující gmSPI-2 (Galleria mellonella silk proteinase inhibitor) byly připraveny pro transformaci hrachu pomocí Agrobakteria. (cs)
  • A set of Czech pea cultivars was genetically modified with genes effecting resistance to pests and pathogens. Three constructs containing gmSPI-2 gene (Galleria mellonella silk proteinase inhibitor) were prepared for Agrobacterium-mediated transformation. Three types of regeneration system of pea derived from mature seeds (induction of trimmed seeds -- TS, cotyledonary nodes -- CN), and immature embryos (IE) were used for application of transformation protocols based on work of Švábová et al. (2005, 2007). After cocultivation, selection and regeneration steps, the potentially transformed plants were cultivated ex vitro, acclimatized and planted in a culture room to yield seed generation T1. Samples of leaf tissues were taken to test gus and bar gene activities histochemically, and then by molecular methods (PCR).
  • A set of Czech pea cultivars was genetically modified with genes effecting resistance to pests and pathogens. Three constructs containing gmSPI-2 gene (Galleria mellonella silk proteinase inhibitor) were prepared for Agrobacterium-mediated transformation. Three types of regeneration system of pea derived from mature seeds (induction of trimmed seeds -- TS, cotyledonary nodes -- CN), and immature embryos (IE) were used for application of transformation protocols based on work of Švábová et al. (2005, 2007). After cocultivation, selection and regeneration steps, the potentially transformed plants were cultivated ex vitro, acclimatized and planted in a culture room to yield seed generation T1. Samples of leaf tissues were taken to test gus and bar gene activities histochemically, and then by molecular methods (PCR). (en)
Title
  • Transformation of Pea to Induce Resistance to Insect Pests and Fungal Pathogens - First Partial Results
  • Transformation of Pea to Induce Resistance to Insect Pests and Fungal Pathogens - First Partial Results (en)
  • Transformace hrachu pro indukci resitence k hmyzím škůdcům a houbovým patogenům - první dílčí výsledky (cs)
skos:prefLabel
  • Transformation of Pea to Induce Resistance to Insect Pests and Fungal Pathogens - First Partial Results
  • Transformation of Pea to Induce Resistance to Insect Pests and Fungal Pathogens - First Partial Results (en)
  • Transformace hrachu pro indukci resitence k hmyzím škůdcům a houbovým patogenům - první dílčí výsledky (cs)
skos:notation
  • RIV/62156489:43210/07:00117835!RIV08-MSM-43210___
http://linked.open.../vavai/riv/strany
  • 24;30
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(1M06030)
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 455543
http://linked.open...ai/riv/idVysledku
  • RIV/62156489:43210/07:00117835
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • pea; transforamtion; silk proteinase inhibitor (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...ontrolniKodProRIV
  • [794D8DD62F41]
http://linked.open...i/riv/mistoVydani
  • Brno
http://linked.open...i/riv/nazevZdroje
  • Funkční genomika a proteomika ve šlechtění rostlin
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...iv/tvurceVysledku
  • Griga, Miroslav
  • Hanáček, Pavel
  • Horáček, Jiří
  • Reinöhl, Vilém
  • Švábová, Lenka
number of pages
http://purl.org/ne...btex#hasPublisher
  • Mendelova zemědělská a lesnická univerzita v Brně
https://schema.org/isbn
  • 978-80-7375-090-9
http://localhost/t...ganizacniJednotka
  • 43210
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