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  • In plants, beta-glucosidases have been implicated in regulating several aspects of development, including phytohormone activation, degradation of endosperm cell wall during germination, and pathogen defense. beta-glucosidases are a widespread group of enzymes that hydrolyze a broad variety of glucosides including aryl- and alkyl-beta-D-glucosides. The maize beta-glucosidase Zm-p60.1 is important for the regulation of plant development through its role in the targeted release of free cytokinins from cytokinin-O-glucosides, their inactive storage forms. For research focused on beta-glucosidase Zm-p60.1 substrate specificity we needed a procedure proving structure stability of the enzyme during mutagenesis. We developed a directed mutagenesis assay using a system comprising specially designed multi degenerated primers and direct screening of colonies containing positive mutations. This method performed in cycles is a suitable tool for directed evolution of enzymes concretely beta-glucosidases i
  • In plants, beta-glucosidases have been implicated in regulating several aspects of development, including phytohormone activation, degradation of endosperm cell wall during germination, and pathogen defense. beta-glucosidases are a widespread group of enzymes that hydrolyze a broad variety of glucosides including aryl- and alkyl-beta-D-glucosides. The maize beta-glucosidase Zm-p60.1 is important for the regulation of plant development through its role in the targeted release of free cytokinins from cytokinin-O-glucosides, their inactive storage forms. For research focused on beta-glucosidase Zm-p60.1 substrate specificity we needed a procedure proving structure stability of the enzyme during mutagenesis. We developed a directed mutagenesis assay using a system comprising specially designed multi degenerated primers and direct screening of colonies containing positive mutations. This method performed in cycles is a suitable tool for directed evolution of enzymes concretely beta-glucosidases i (en)
  • Byla vyvinuta nová metoda pro řízenou evoluci proteinů. Metoda je testována na rostlinné beta glukozidáze Zm-p60.1, která je důležitá pro svou schopnost uvolňovat rostlinné hormony z glukokonjugátů. (cs)
Title
  • Improved method for directed enzyme evolution
  • Improved method for directed enzyme evolution (en)
  • Vylepšená metoda pro řízenou evoluci enzymů (cs)
skos:prefLabel
  • Improved method for directed enzyme evolution
  • Improved method for directed enzyme evolution (en)
  • Vylepšená metoda pro řízenou evoluci enzymů (cs)
skos:notation
  • RIV/62156489:43210/07:00117762!RIV08-MSM-43210___
http://linked.open.../vavai/riv/strany
  • 108;108
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(LC06034)
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
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http://linked.open...iv/duvernostUdaju
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  • 425766
http://linked.open...ai/riv/idVysledku
  • RIV/62156489:43210/07:00117762
http://linked.open...riv/jazykVysledku
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  • directed enzyme evolution; x-glu; beta-glucosidase (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...ontrolniKodProRIV
  • [EDCA67FBBF20]
http://linked.open...v/mistoKonaniAkce
  • Brno
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  • MendelNet'07 Agro
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http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...iv/tvurceVysledku
  • Janda, Lubomír
  • Brzobohatý, Břetislav
  • Mazura, Pavel
http://linked.open...vavai/riv/typAkce
http://linked.open.../riv/zahajeniAkce
number of pages
http://purl.org/ne...btex#hasPublisher
  • Mendelova zemědělská a lesnická univerzita v Brně
https://schema.org/isbn
  • 978-80-7375-119-7
http://localhost/t...ganizacniJednotka
  • 43210
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