About: Detection of metallothionein level at transgenic plants of tobacco

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Jako modelová rostlina v této práci byl vybrán tabák (Nicotiana tabacum) transformovaný pomocí Agrobacterium tumefaciens. Konstrukt byl schopný exprimovat kvasinkový metalothionein.. Předmětem zájmu bylo stanovení množství exprimovanéo metalothioneinu v rostlinných tkáních. Pro tento účel jsem použily optimalizovanou techniku nazvanou adsorpční přenosová technika spojená s diferenční pulsní voltametrií- Brdičkova reakce. Zjistil jsme, že rostlin syntetizovali rozdílná množství metalothioneinu. (cs) The model plant in this work tobacco (Nicotiana tabacum) was chosen to be transformed by Agrobacterium tumefaciens (Macek et al., 2002). The construct were then able to express yeast metallothionein gene. Nevertheless we were interested in the issue, if we would be able to quantify the expressed MTs. For these purposes we suggested and optimized electrochemical method called adsorptive transfer stripping technique coupled with differential pulse voltammetry -- Brdicka reaction (Petrlova et al., 2006). We found out that plant were able to synthesized from tens to hundreds ng MT per g of fresh weight. The model plant in this work tobacco (Nicotiana tabacum) was chosen to be transformed by Agrobacterium tumefaciens (Macek et al., 2002). The construct were then able to express yeast metallothionein gene. Nevertheless we were interested in the issue, if we would be able to quantify the expressed MTs. For these purposes we suggested and optimized electrochemical method called adsorptive transfer stripping technique coupled with differential pulse voltammetry -- Brdicka reaction (Petrlova et al., 2006). We found out that plant were able to synthesized from tens to hundreds ng MT per g of fresh weight. (en)
Title	Detection of metallothionein level at transgenic plants of tobacco Detekce úrovně metalothioneinu v transgeních rostlinách tabáků (cs) Detection of metallothionein level at transgenic plants of tobacco (en)
skos:prefLabel	Detection of metallothionein level at transgenic plants of tobacco Detekce úrovně metalothioneinu v transgeních rostlinách tabáků (cs) Detection of metallothionein level at transgenic plants of tobacco (en)
skos:notation	RIV/62156489:43210/07:00110034!RIV08-GA0-43210___
http://linked.open.../vavai/riv/strany	119;119
http://linked.open...avai/riv/aktivita	P S
http://linked.open...avai/riv/aktivity	P(1M06030), P(GA522/07/0692), S
http://linked.open...vai/riv/dodaniDat	2008
http://linked.open...aciTvurceVysledku	Havel, Ladislav Křížková, Soňa Šupálková, Veronika Diopan, Václav Adam, Vojtěch Zehnálek, Josef Kizek, René Baloun, Jiří Shestivska, Violetta
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Mendelova univerzita v Brně / Agronomická fakulta
http://linked.open...dnocenehoVysledku	416538
http://linked.open...ai/riv/idVysledku	RIV/62156489:43210/07:00110034
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	transgenic plant; electrochemical detection (en)
http://linked.open.../riv/klicoveSlovo	electrochemical detection transgenic plant
http://linked.open...ontrolniKodProRIV	[61379AE88D16]
http://linked.open...v/mistoKonaniAkce	Vysoké Tatry, Slovenská republika
http://linked.open...i/riv/nazevZdroje	Recent advanced in plant biotechnology
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	9 (xsd:int)
http://linked.open...cetTvurcuVysledku	12 (xsd:int)
http://linked.open...vavai/riv/projekt	Study of thiol compounds production at plants - use in phytoremediation technologies Functional genomics and proteomics for crop improvement
http://linked.open...UplatneniVysledku	2007
http://linked.open...iv/tvurceVysledku	Adam, Vojtěch Havel, Ladislav Kizek, René Křížková, Soňa Kotrba, P. Macek, Tomáš Zehnálek, Josef Šupálková, Veronika Shestivska, Violetta Mackova, Martina Baloun, Jiří Diopan, Václav
http://linked.open...vavai/riv/typAkce	WRD - Světová
http://linked.open.../riv/zahajeniAkce	2007-01-01 (xsd:date)
number of pages	1 (xsd:int)
http://purl.org/ne...btex#hasPublisher	Neuveden
https://schema.org/isbn	978-80-89088-51-5
http://localhost/t...ganizacniJednotka	43210

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