About: Rapid-Resolution HPLC with Spectrometric Detection for the Determination and Identification of Isoflavones in Soy Preparations and Plant Extracts

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	A rapid-resolution HPLC/UV-VIS DAD separation method (which takes < 1 min) for the determination and identification of genistin, genistein, daidzein, daidzin, glycitin, glycitein, ononin, formononetin, sissotrin and biochanin A in fmol quantities in submicroliter sample volumes was optimized. A linear gradient elution (0 min 22% B, 1.0 min 80% B, 1.4 min 100% B, 1.8 min 22% B) using a mobile phase containing 0.2 % (v/v) acetic acid (solvent A) and methanol (solvent B) was applied on a Zorbax SB C-18 column (1.8 mu m particle size) at 80 degrees C. The method was verified using samples of bits of soy and methanolic extracts from Trifolium pratense, Iresine herbstii and Ononis spinosa plants. Pseudobaptigenin glucoside, irilone, prunetin, texasin, tlatlancuayin and other isoflavones, in addition to aglycones of isoflavones and their beta-glucosides and malonyl and acetyl derivatives, were identified by UV-VIS DAD and electrospray mass spectrometric (ESI-MS) detection in the extracts. A rapid-resolution HPLC/UV-VIS DAD separation method (which takes < 1 min) for the determination and identification of genistin, genistein, daidzein, daidzin, glycitin, glycitein, ononin, formononetin, sissotrin and biochanin A in fmol quantities in submicroliter sample volumes was optimized. A linear gradient elution (0 min 22% B, 1.0 min 80% B, 1.4 min 100% B, 1.8 min 22% B) using a mobile phase containing 0.2 % (v/v) acetic acid (solvent A) and methanol (solvent B) was applied on a Zorbax SB C-18 column (1.8 mu m particle size) at 80 degrees C. The method was verified using samples of bits of soy and methanolic extracts from Trifolium pratense, Iresine herbstii and Ononis spinosa plants. Pseudobaptigenin glucoside, irilone, prunetin, texasin, tlatlancuayin and other isoflavones, in addition to aglycones of isoflavones and their beta-glucosides and malonyl and acetyl derivatives, were identified by UV-VIS DAD and electrospray mass spectrometric (ESI-MS) detection in the extracts. (en)
Title	Rapid-Resolution HPLC with Spectrometric Detection for the Determination and Identification of Isoflavones in Soy Preparations and Plant Extracts Rapid-Resolution HPLC with Spectrometric Detection for the Determination and Identification of Isoflavones in Soy Preparations and Plant Extracts (en)
skos:prefLabel	Rapid-Resolution HPLC with Spectrometric Detection for the Determination and Identification of Isoflavones in Soy Preparations and Plant Extracts Rapid-Resolution HPLC with Spectrometric Detection for the Determination and Identification of Isoflavones in Soy Preparations and Plant Extracts (en)
skos:notation	RIV/62156489:43210/07:00110002!RIV12-GA0-43210___
http://linked.open...avai/riv/aktivita	P V
http://linked.open...avai/riv/aktivity	P(GA525/07/0338), V
http://linked.open...iv/cisloPeriodika	7-8
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Benešová, Lucie Kubáň, Vlastimil Vacek, Jan Klejdus, Bořivoj
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Mendelova univerzita v Brně / Agronomická fakulta
http://linked.open...dnocenehoVysledku	446429
http://linked.open...ai/riv/idVysledku	RIV/62156489:43210/07:00110002
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	isoflavone identification; hplc; plant extracts; fast chromatography; isoflavones (en)
http://linked.open.../riv/klicoveSlovo	plant extracts hplc isoflavone identification fast chromatography isoflavones
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[A63A7E92B2DF]
http://linked.open...i/riv/nazevZdroje	Analytical and Bioanalytical Chemistry
http://linked.open...in/vavai/riv/obor	CB
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Isoflavonoids in algae and cyanobacteria
http://linked.open...UplatneniVysledku	2007
http://linked.open...v/svazekPeriodika	389
http://linked.open...iv/tvurceVysledku	Benešová, Lucie Kubáň, Vlastimil Kopecký, Jan Lapčík, Oldřich Vacek, Jan Klejdus, Bořivoj
issn	1618-2642
number of pages	9 (xsd:int)
http://localhost/t...ganizacniJednotka	43210

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