About: The Transformation of Pea (Pisum sativum L.) : Applicable Methods of Agrobacterium tumefaciens-mediated Gene Transfer

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Three methods of transformation of pea (Pisum sativum ssp. sativum L. var. medullare) were tested. The most efficient Agrobacterium tumefaciens-mediated T-DNA transfer was obtained using embryonic segments from mature pea seeds as initial explants. The transformation procedure was based on the transfer of the T-DNA region with the reporter gene uidA and selection gene bar. The expression of beta-glucuronidase (GUS) in the regenerated shoots was tested using the histochemical method and the shoots were selected on a medium containing phosphinothricin (PPT). The shoots of putative transformants were rooted and transferred to non-sterile conditions. Transient expression of the uidA gene in the tissues after co-cultivation and in the course of short-term shoot cultivation (confirmed by histochemical analysis of GUS and by RT-PCR of mRNA) was achieved; however, we have not yet succeeded in proving stable incorporation of the transgene in the analysed plants. Three methods of transformation of pea (Pisum sativum ssp. sativum L. var. medullare) were tested. The most efficient Agrobacterium tumefaciens-mediated T-DNA transfer was obtained using embryonic segments from mature pea seeds as initial explants. The transformation procedure was based on the transfer of the T-DNA region with the reporter gene uidA and selection gene bar. The expression of beta-glucuronidase (GUS) in the regenerated shoots was tested using the histochemical method and the shoots were selected on a medium containing phosphinothricin (PPT). The shoots of putative transformants were rooted and transferred to non-sterile conditions. Transient expression of the uidA gene in the tissues after co-cultivation and in the course of short-term shoot cultivation (confirmed by histochemical analysis of GUS and by RT-PCR of mRNA) was achieved; however, we have not yet succeeded in proving stable incorporation of the transgene in the analysed plants. (en) Byly testovány tři metody transformace hrachu (Pisum sativum ssp. sativum L. var. medullare). (cs)
Title	The Transformation of Pea (Pisum sativum L.) : Applicable Methods of Agrobacterium tumefaciens-mediated Gene Transfer The Transformation of Pea (Pisum sativum L.) : Applicable Methods of Agrobacterium tumefaciens-mediated Gene Transfer (en) Transformace hrachu (Pisum sativum L.): Použitelné metody přenosu genů pomocí Agrobacterium tumefaciens (cs)
skos:prefLabel	The Transformation of Pea (Pisum sativum L.) : Applicable Methods of Agrobacterium tumefaciens-mediated Gene Transfer The Transformation of Pea (Pisum sativum L.) : Applicable Methods of Agrobacterium tumefaciens-mediated Gene Transfer (en) Transformace hrachu (Pisum sativum L.): Použitelné metody přenosu genů pomocí Agrobacterium tumefaciens (cs)
skos:notation	RIV/62156489:43210/07:00094903!RIV08-MZE-43210___
http://linked.open.../vavai/riv/strany	157;163
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(QF3072)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2008
http://linked.open...aciTvurceVysledku	Matušková, Petra Procházka, Stanislav Hanáček, Pavel Krejčí, Petra Reinöhl, Vilém
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Mendelova univerzita v Brně / Agronomická fakulta
http://linked.open...dnocenehoVysledku	455542
http://linked.open...ai/riv/idVysledku	RIV/62156489:43210/07:00094903
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Agrobacterium tumefaciens; pea; transformation (en)
http://linked.open.../riv/klicoveSlovo	Agrobacterium tumefaciens pea transformation
http://linked.open...odStatuVydavatele	PL - Polská republika
http://linked.open...ontrolniKodProRIV	[38C3DAF5D7DB]
http://linked.open...i/riv/nazevZdroje	Acta Physiologiae Plantarum
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Development of transgenic pea (Pisum sativum L.) lines with enhanced resistance to viral pathogens.
http://linked.open...UplatneniVysledku	2007
http://linked.open...v/svazekPeriodika	29
http://linked.open...iv/tvurceVysledku	Hanáček, Pavel Procházka, Stanislav Reinöhl, Vilém Matušková, Petra Krejčí, Petra
issn	0137-5881
number of pages	7 (xsd:int)
http://localhost/t...ganizacniJednotka	43210

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