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| Description
| - Cytokinins (CKs) regulate various developmental processes in plants. The objective of our study was to analyze changes in the Arabidopsis proteome caused by increase in endogenous CKs which was achieved by expressing a CK biosynthesis gene, ipt, for 15 days. Image analysis of 2-D gels revealed differences in protein expression between plants with elevated CK content and controls. Differentially expressed proteins were identified by MALDI-TOF MS, and a majority of them were related to oxidative stress and defense mechanisms including jasmonic acid (JA) inducible proteins. Subsequent analysis revealed increased levels of H2O2 and JA in seedlings with elevated CKs. For the first time, the data indicate a link between CKs, JA and oxidative stress at the proteome level.
- Cytokinins (CKs) regulate various developmental processes in plants. The objective of our study was to analyze changes in the Arabidopsis proteome caused by increase in endogenous CKs which was achieved by expressing a CK biosynthesis gene, ipt, for 15 days. Image analysis of 2-D gels revealed differences in protein expression between plants with elevated CK content and controls. Differentially expressed proteins were identified by MALDI-TOF MS, and a majority of them were related to oxidative stress and defense mechanisms including jasmonic acid (JA) inducible proteins. Subsequent analysis revealed increased levels of H2O2 and JA in seedlings with elevated CKs. For the first time, the data indicate a link between CKs, JA and oxidative stress at the proteome level. (en)
- Cytokininy (CK) regulují řadu vývojových procesů v rostlinách. Cílem naší práce bylo analyzovat změny proteomu A. thaliana způsobené zvýšením endogenních CK, čehož jsme dosáhli expresí genu biosyntézy CK, ipt, po dobu 15 dnů. Analýza obrazu 2-D gelů ukázala rozdíly v expresi proteinů mezi rostlinami se zvýšenými CK a kontrolami. Rozdílně exprimované proteiny byly identifikovány MALDI-TOF MS, přičemž většina z nich měla souvislost s oxidativním stresem a obrannými mechanismy, včetně proteinů indukovatelných kyselinou jasmonovou (JA). Následné analýzy ukázaly zvýšené hladiny H2O2 a JA v semenáčcích se zvýšenými CK. Data tak poprvé naznačují vztah mezi CK, JA a oxidativním stresem na úrovni proteomu. (cs)
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| Title
| - Proteome Analysis Indicates a Role of Oxidative Stress in Cytokinin Action in Arabidopsis Seedlings
- Proteomická analýza semenáčků Arabidopsis naznačuje úlohu oxidativního stresu v působení cytokininů (cs)
- Proteome Analysis Indicates a Role of Oxidative Stress in Cytokinin Action in Arabidopsis Seedlings (en)
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| skos:prefLabel
| - Proteome Analysis Indicates a Role of Oxidative Stress in Cytokinin Action in Arabidopsis Seedlings
- Proteomická analýza semenáčků Arabidopsis naznačuje úlohu oxidativního stresu v působení cytokininů (cs)
- Proteome Analysis Indicates a Role of Oxidative Stress in Cytokinin Action in Arabidopsis Seedlings (en)
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| skos:notation
| - RIV/62156489:43210/06:00104612!RIV08-MSM-43210___
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/62156489:43210/06:00104612
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - cytokinin; Arabidopsis thaliana; proteome; oxidative stress (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/mistoVydani
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| http://linked.open...i/riv/nazevZdroje
| - MendelNet'06 Agro - Proceedings of International Ph.D. Students Conference
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...iv/tvurceVysledku
| - Malbeck, Jiří
- Novák, Jan
- Novák, Ondřej
- Brzobohatý, Břetislav
- Váňová, Pavlína
- Chevalier, François
- Rossignol, Michel
- Rofidal, Valérie
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| number of pages
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| http://purl.org/ne...btex#hasPublisher
| - Mendelova zemědělská a lesnická univerzita v Brně
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| https://schema.org/isbn
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| http://localhost/t...ganizacniJednotka
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