| Attributes | Values |
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| rdf:type
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| Description
| - Bylo zjištěno, že analýza obrazu je vhodnou metodou pro sledování růstu kultur raných somatických embryí smrku ztepilého (Picea abies /L./ Karst.). Obdobně je možné využít analýzu obrazu při sledování živatoschopnosti s využitím fluorescenčních barviv. Získané metody byly využity při sledování vlivu Pb-EDTA na raná soamtická embrya. (cs)
- Image analysis (IA) was used to determine the areas and circumferences of clusters of early somatic embryos (ESEs) of the Norway spruce (Picea abies /L./Karst.). Results obtained from IA were compared with the fresh weights of the ESE clusters and their esterase activities. The areas of the ESE clusters correlated well with both the increases in fresh weight (R2=0.99) of the ESEs and their esterase activities (R2=0.99). In addition, we studied the viability of the ESEs, which was determined by (a) double staining with fluorescein diacetate and propidium iodide (the resulting fluorescence was quantified by IA) and (b) determining esterase activity using a spectrofluorimetric detector. The results obtained with IA and esterase assay were comparable (the deviation between the tangents of the bisectors was 6.4%). IA was also used to study the effect of Pb--EDTA
- Image analysis (IA) was used to determine the areas and circumferences of clusters of early somatic embryos (ESEs) of the Norway spruce (Picea abies /L./Karst.). Results obtained from IA were compared with the fresh weights of the ESE clusters and their esterase activities. The areas of the ESE clusters correlated well with both the increases in fresh weight (R2=0.99) of the ESEs and their esterase activities (R2=0.99). In addition, we studied the viability of the ESEs, which was determined by (a) double staining with fluorescein diacetate and propidium iodide (the resulting fluorescence was quantified by IA) and (b) determining esterase activity using a spectrofluorimetric detector. The results obtained with IA and esterase assay were comparable (the deviation between the tangents of the bisectors was 6.4%). IA was also used to study the effect of Pb--EDTA (en)
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| Title
| - Application of computer imaging, stripping voltammetry and mass spectrometry for study of the effect of lead (Pb-EDTA) on growth and viability of early somatic embryos of Norway spruce (Picea abies /L./ Karst.)
- Application of computer imaging, stripping voltammetry and mass spectrometry for study of the effect of lead (Pb-EDTA) on growth and viability of early somatic embryos of Norway spruce (Picea abies /L./ Karst.) (en)
- Využití polčítačového zpracovnán obrazu, rozpouštěcí voltametrie a hmotnostní spektroskopie při studiu vlivu olova na růst a životaschopnost raných soamtických embryí smrku ztepilého (Picea abies /L./ Karst.) (cs)
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| skos:prefLabel
| - Application of computer imaging, stripping voltammetry and mass spectrometry for study of the effect of lead (Pb-EDTA) on growth and viability of early somatic embryos of Norway spruce (Picea abies /L./ Karst.)
- Application of computer imaging, stripping voltammetry and mass spectrometry for study of the effect of lead (Pb-EDTA) on growth and viability of early somatic embryos of Norway spruce (Picea abies /L./ Karst.) (en)
- Využití polčítačového zpracovnán obrazu, rozpouštěcí voltametrie a hmotnostní spektroskopie při studiu vlivu olova na růst a životaschopnost raných soamtických embryí smrku ztepilého (Picea abies /L./ Karst.) (cs)
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| skos:notation
| - RIV/62156489:43210/05:00056375!RIV06-GA0-43210___
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...iv/cisloPeriodika
| |
| http://linked.open...vai/riv/dodaniDat
| |
| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
| |
| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/62156489:43210/05:00056375
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| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - Norway spruce; computer imaging; mass spectrometry; somatic embryogenesis; stripping voltammetry (en)
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| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...odStatuVydavatele
| - DE - Spolková republika Německo
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| http://linked.open...ontrolniKodProRIV
| |
| http://linked.open...i/riv/nazevZdroje
| - Analytical and bioanalytical chemistry
|
| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...vavai/riv/projekt
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...v/svazekPeriodika
| |
| http://linked.open...iv/tvurceVysledku
| - Adam, Vojtěch
- Havel, Ladislav
- Vlašínová, Helena
- Klejdus, Bořivoj
- Víteček, Jan
- Petřek, Jiří
- Kízek, René
- Kramer, Karl J.
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| issn
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| number of pages
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| http://localhost/t...ganizacniJednotka
| |
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