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  • The article is in English. See original desription in the original language. (en)
  • We describe the multi-step regeneration system of medicinal plant Schisandra chinensis (Turcz.) Baill. The seeds were pre-treated with 0.005 microM thidiazuron. Subsequently the zygotic embryos of the early heart stage were cultured on medium with 50 microM of 2,4-dichlorophenoxyacetic acid (2,4-D) and after three weeks the embryogenic calli were transferred to a medium with 10 microM of 2,4-D and 4 microM of 6-benzyladenine and were sub-cultured at the 4-week intervals. Abscisic acid (30 microM) and polyethyleneglycol (3 %) significantly influenced the synchronization of development of the somatic embryos (SEs) to the globular stage. The following culture on a medium without growth regulators resulted in full developed cotyledonary stage SEs. Indole-3-butyric acid (0.05 microM)conntributed to their rapid conversion to plantlets.
  • We describe the multi-step regeneration system of medicinal plant Schisandra chinensis (Turcz.) Baill. The seeds were pre-treated with 0.005 microM thidiazuron. Subsequently the zygotic embryos of the early heart stage were cultured on medium with 50 microM of 2,4-dichlorophenoxyacetic acid (2,4-D) and after three weeks the embryogenic calli were transferred to a medium with 10 microM of 2,4-D and 4 microM of 6-benzyladenine and were sub-cultured at the 4-week intervals. Abscisic acid (30 microM) and polyethyleneglycol (3 %) significantly influenced the synchronization of development of the somatic embryos (SEs) to the globular stage. The following culture on a medium without growth regulators resulted in full developed cotyledonary stage SEs. Indole-3-butyric acid (0.05 microM)conntributed to their rapid conversion to plantlets. (cs)
Title
  • Somatic embryogenesis from zygotic embryos of Schisandra chinensis
  • Somatic embryogenesis from zygotic embryos of Schisandra chinensis (en)
  • Somatic embryogenesis from zygotic embryos of Schisandra chinensis (cs)
skos:prefLabel
  • Somatic embryogenesis from zygotic embryos of Schisandra chinensis
  • Somatic embryogenesis from zygotic embryos of Schisandra chinensis (en)
  • Somatic embryogenesis from zygotic embryos of Schisandra chinensis (cs)
skos:notation
  • RIV/62156489:43210/05:00004744!RIV06-GA0-43210___
http://linked.open.../vavai/riv/strany
  • 451;454
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GA521/02/1129)
http://linked.open...iv/cisloPeriodika
  • 3
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 543480
http://linked.open...ai/riv/idVysledku
  • RIV/62156489:43210/05:00004744
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • abscisic acied; maturation; medicinal plant; micropropagation; somatic embryo conversion (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • CZ - Česká republika
http://linked.open...ontrolniKodProRIV
  • [0B5186E38F21]
http://linked.open...i/riv/nazevZdroje
  • Biologia Plantarum
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 49
http://linked.open...iv/tvurceVysledku
  • Havel, Ladislav
  • Vlašínová, Helena
  • Smíšková, Alexandra
issn
  • 0006-3134
number of pages
http://localhost/t...ganizacniJednotka
  • 43210
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