About: High performance liquid chromatography and capillary electrophoresis determination of sanguinarine in biological matrices

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	HPLC and CE methods were employed to determine the quaternary benzo[c]phenanthridine alkaloid sanguinarine,in biological matrices (rat hepatocytes, human gingival fibroblasts, feed, porcine faeces, body fluids and tissues). HPLC was carried out on a C-18column using gradient elution and ion pairing techniques with 1-heptanesulfonic acid as ion pairing agent under acidic conditions. The detection limit for fluorimetric detection at lambda(ex) = 327 nm and lambda(em) = 577 nm was 3 nM sanguinarine. CE an alyses were performed in 50 mM phosphate-Na buffer pH 2.5, with 150 mM SDS used for pre-concentration by the sweeping effect. This experimental configuration allows injecting the total capillary length with sanguinarine sample. The detection limit for UVdetection at 285 nm was 12 nM. Both methods are suitable for analysing submicromolar quantities of sanguinarine in biological materials. The HPLC method is more sensitive than CE because it uses fluorescence detection. HPLC and CE methods were employed to determine the quaternary benzo[c]phenanthridine alkaloid sanguinarine,in biological matrices (rat hepatocytes, human gingival fibroblasts, feed, porcine faeces, body fluids and tissues). HPLC was carried out on a C-18column using gradient elution and ion pairing techniques with 1-heptanesulfonic acid as ion pairing agent under acidic conditions. The detection limit for fluorimetric detection at lambda(ex) = 327 nm and lambda(em) = 577 nm was 3 nM sanguinarine. CE an alyses were performed in 50 mM phosphate-Na buffer pH 2.5, with 150 mM SDS used for pre-concentration by the sweeping effect. This experimental configuration allows injecting the total capillary length with sanguinarine sample. The detection limit for UVdetection at 285 nm was 12 nM. Both methods are suitable for analysing submicromolar quantities of sanguinarine in biological materials. The HPLC method is more sensitive than CE because it uses fluorescence detection. (en)
Title	High performance liquid chromatography and capillary electrophoresis determination of sanguinarine in biological matrices High performance liquid chromatography and capillary electrophoresis determination of sanguinarine in biological matrices (en)
skos:prefLabel	High performance liquid chromatography and capillary electrophoresis determination of sanguinarine in biological matrices High performance liquid chromatography and capillary electrophoresis determination of sanguinarine in biological matrices (en)
skos:notation	RIV/61989592:15410/03:00001414!RIV/2004/MSM/154104/N
http://linked.open.../vavai/riv/strany	679-685
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(MSM 151100003), Z(MSM 153100013)
http://linked.open...iv/cisloPeriodika	8
http://linked.open...vai/riv/dodaniDat	2004
http://linked.open...aciTvurceVysledku	Walterová, Daniela Barták, Petr Ulrichová, Jitka Bednář, Petr Ševčík, Juraj Kosina, Pavel Modrianský, Martin
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Palackého v Olomouci / Pedagogická fakulta
http://linked.open...dnocenehoVysledku	608799
http://linked.open...ai/riv/idVysledku	RIV/61989592:15410/03:00001414
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	SAMPLE SELF-STACKING; ZONE-ELECTROPHORESIS; BENZOPHENANTHRIDINE ALKALOIDS; FLUOROMETRIC DETECTION; SEPARATION; ISOTACHOPHORESIS; CHARGE; HPLC (en)
http://linked.open.../riv/klicoveSlovo	SEPARATION ZONE-ELECTROPHORESIS HPLC ISOTACHOPHORESIS CHARGE BENZOPHENANTHRIDINE ALKALOIDS FLUOROMETRIC DETECTION SAMPLE SELF-STACKING
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[9DE9389181F5]
http://linked.open...i/riv/nazevZdroje	Journal of Separation Science
http://linked.open...in/vavai/riv/obor	CB
http://linked.open...ichTvurcuVysledku	7 (xsd:int)
http://linked.open...cetTvurcuVysledku	8 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...UplatneniVysledku	2003
http://linked.open...v/svazekPeriodika	26
http://linked.open...iv/tvurceVysledku	Barták, Petr Bednář, Petr Kosina, Pavel Ulrichová, Jitka Ševčík, Juraj Modrianský, Martin Walterová, Daniela Gavenda, A.
http://linked.open...n/vavai/riv/zamer	http://linked.opendata.cz/resource/domain/vavai/zamer/MSM%20151100003 http://linked.opendata.cz/resource/domain/vavai/zamer/MSM%20153100013
issn	1615-9306
number of pages	7 (xsd:int)
http://localhost/t...ganizacniJednotka	15410

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