About: Quantification of Stress-Induced Mitogen-Activated Protein Kinase Expressional Dynamic Using Reverse Transcription Quantitative Real-Time PCR

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Although it is generally accepted that signal transduction in plant mitogen-activated protein kinase signaling cascades is regulated via rapid posttranslational modifications, there are also several compelling examples of swift stress induced transcriptional activation of plant MAP kinase genes. A possible function of these fast and transient events is to compensate for protein losses caused by degradation of phosphorylated MAP kinases within stimulated pathways. Nevertheless, there is still need for additional evidence to precisely describe the regulatory role of plant MAP kinase transcriptional dynamics, especially in the context of whole stress stimulated pathways including also other signaling molecules and transcription factors. During the last two decades a reverse transcription quantitative real-time PCR became a golden choice for the accurate and fast quantification of the gene expression and gene expression dynamic. In here, we provide a robust, cost-effective SYBR Green-based RT-qPCR protocol that is suitable for the quantification of stress induced plant MAP kinase transcriptional dynamics in various plant species. Although it is generally accepted that signal transduction in plant mitogen-activated protein kinase signaling cascades is regulated via rapid posttranslational modifications, there are also several compelling examples of swift stress induced transcriptional activation of plant MAP kinase genes. A possible function of these fast and transient events is to compensate for protein losses caused by degradation of phosphorylated MAP kinases within stimulated pathways. Nevertheless, there is still need for additional evidence to precisely describe the regulatory role of plant MAP kinase transcriptional dynamics, especially in the context of whole stress stimulated pathways including also other signaling molecules and transcription factors. During the last two decades a reverse transcription quantitative real-time PCR became a golden choice for the accurate and fast quantification of the gene expression and gene expression dynamic. In here, we provide a robust, cost-effective SYBR Green-based RT-qPCR protocol that is suitable for the quantification of stress induced plant MAP kinase transcriptional dynamics in various plant species. (en)
Title	Quantification of Stress-Induced Mitogen-Activated Protein Kinase Expressional Dynamic Using Reverse Transcription Quantitative Real-Time PCR Quantification of Stress-Induced Mitogen-Activated Protein Kinase Expressional Dynamic Using Reverse Transcription Quantitative Real-Time PCR (en)
skos:prefLabel	Quantification of Stress-Induced Mitogen-Activated Protein Kinase Expressional Dynamic Using Reverse Transcription Quantitative Real-Time PCR Quantification of Stress-Induced Mitogen-Activated Protein Kinase Expressional Dynamic Using Reverse Transcription Quantitative Real-Time PCR (en)
skos:notation	RIV/61989592:15310/14:33153248!RIV15-MSM-15310___
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(GPP501/12/P455), P(LO1204)
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Křenek, Pavel Smékalová, Veronika
http://linked.open.../riv/druhVysledku	C - Kapitola v knize
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Palackého v Olomouci / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	41072
http://linked.open...ai/riv/idVysledku	RIV/61989592:15310/14:33153248
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	stress; transcript level; RT-qPCR; expression; Mitogen-Activated Protein Kinase (en)
http://linked.open.../riv/klicoveSlovo	Mitogen-Activated Protein Kinase expression stress RT-qPCR transcript level
http://linked.open...ontrolniKodProRIV	[4A7E3A040AA9]
http://linked.open...i/riv/mistoVydani	Clifton
http://linked.open...vEdiceCisloSvazku	Methods in Molecular Biology 1171
http://linked.open...i/riv/nazevZdroje	Plant MAP Kinases: Methods and Protocols
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...v/pocetStranKnihy	266 (xsd:int)
http://linked.open...cetTvurcuVysledku	2 (xsd:int)
http://linked.open...vavai/riv/projekt	The role of mitogen-activated protein kinases (MPKs) in barley responses to Puccinia hordei attack Udržitelný rozvoj výzkumu v Centru regionu Haná
http://linked.open...UplatneniVysledku	2014
http://linked.open...iv/tvurceVysledku	Křenek, Pavel Smékalová, Veronika
number of pages	13 (xsd:int)
http://bibframe.org/vocab/doi	10.1007/978-1-4939-0922-3_2
http://purl.org/ne...btex#hasPublisher	Humana Press
https://schema.org/isbn	978-1-4939-0921-6
http://localhost/t...ganizacniJednotka	15310

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