About: Dominance of Methanosarcinales Phylotypes and Depth-Wise Distribution of Methanogenic Community in Fresh Water Sediments of Sitka Stream from Czech Republic

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About: Dominance of Methanosarcinales Phylotypes and Depth-Wise Distribution of Methanogenic Community in Fresh Water Sediments of Sitka Stream from Czech Republic Goto Sponge NotDistinct Permalink

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rdfs:seeAlso	http://download.springer.com/static/pdf/368/art%253A10.1007%252Fs00284-014-0659-8.pdf?auth66=1425291013_36c3ff24ed4b99c1445b6a6e3b172c26&ext=.pdf
Description	The variation in the diversity of methanogens in sediment depths from Sitka stream was studied by constructing a 16S rRNA gene library using methanogen-specific primers and a denaturing gradient gel electrophoresis (DGGE)-based approach. A total of nine different phylotypes from the 16S rRNA library were obtained, and all of them were clustered within the order Methanosarcinales. These nine phylotypes likely represent nine new species and at least 5-6 new genera. Similarly, DGGE analysis revealed an increase in the diversity of methanogens with an increase in sediment depth. These results suggest that Methanosarcinales phylotypes might be the dominant methanogens in the sediment from Sitka stream, and the diversity of methanogens increases as the depth increases. Results of the present study will help in making effective strategies to monitor the dominant methanogen phylotypes and methane emissions in the environment. The variation in the diversity of methanogens in sediment depths from Sitka stream was studied by constructing a 16S rRNA gene library using methanogen-specific primers and a denaturing gradient gel electrophoresis (DGGE)-based approach. A total of nine different phylotypes from the 16S rRNA library were obtained, and all of them were clustered within the order Methanosarcinales. These nine phylotypes likely represent nine new species and at least 5-6 new genera. Similarly, DGGE analysis revealed an increase in the diversity of methanogens with an increase in sediment depth. These results suggest that Methanosarcinales phylotypes might be the dominant methanogens in the sediment from Sitka stream, and the diversity of methanogens increases as the depth increases. Results of the present study will help in making effective strategies to monitor the dominant methanogen phylotypes and methane emissions in the environment. (en)
Title	Dominance of Methanosarcinales Phylotypes and Depth-Wise Distribution of Methanogenic Community in Fresh Water Sediments of Sitka Stream from Czech Republic Dominance of Methanosarcinales Phylotypes and Depth-Wise Distribution of Methanogenic Community in Fresh Water Sediments of Sitka Stream from Czech Republic (en)
skos:prefLabel	Dominance of Methanosarcinales Phylotypes and Depth-Wise Distribution of Methanogenic Community in Fresh Water Sediments of Sitka Stream from Czech Republic Dominance of Methanosarcinales Phylotypes and Depth-Wise Distribution of Methanogenic Community in Fresh Water Sediments of Sitka Stream from Czech Republic (en)
skos:notation	RIV/61989592:15310/14:33153246!RIV15-MSM-15310___
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(EE2.3.30.0041)
http://linked.open...iv/cisloPeriodika	6
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Rulík, Martin Brablcová, Lenka Buriánková, Iva Bednařík, Adam Chaudhary, Prem Prashant
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Palackého v Olomouci / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	12168
http://linked.open...ai/riv/idVysledku	RIV/61989592:15310/14:33153246
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	soil; lake; rice roots; murhah buffalos; archaeal community; molecular diversity; methanomicrobium phylotype; neighbor-joining method; buffalos Bubalus-bubalis; small lowland stream (en)
http://linked.open.../riv/klicoveSlovo	archaeal community buffalos Bubalus-bubalis methanomicrobium phylotype murhah buffalos neighbor-joining method rice roots small lowland stream molecular diversity lake soil
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[6DA0AFA30ADD]
http://linked.open...i/riv/nazevZdroje	Current Microbiology
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
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http://linked.open...vavai/riv/projekt	Podpora vytváření excelentních výzkumných týmů a intersektorální mobility na Univerzitě Palackého v Olomouci II.
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	69
http://linked.open...iv/tvurceVysledku	Rulík, Martin Bednařík, Adam Brablcová, Lenka Buriánková, Iva Chaudhary, Prem Prashant Wright, Andre-Denis G
http://linked.open...ain/vavai/riv/wos	000343913800006
issn	0343-8651
number of pages	8 (xsd:int)
http://bibframe.org/vocab/doi	10.1007/s00284-014-0659-8
http://localhost/t...ganizacniJednotka	15310

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