About: Integrative Chemical Proteomics and Cell Biology Methods to Study Endocytosis and Vesicular Trafficking in Arabidopsis

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About: Integrative Chemical Proteomics and Cell Biology Methods to Study Endocytosis and Vesicular Trafficking in Arabidopsis Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://link.springer.com/protocol/10.1007%2F978-1-4939-1420-3_20
Description	We present a comprehensive approach combining proteomics and cell biology to study vesicular trafficking in plants. Within this approach, we exploit chemical compounds inhibiting particular vesicular trafficking events in plant cells. Treatment of plants with these relatively specific inhibitors results in intracellular accumulation of proteins being transported by vesicles as well as in a change in abundance of regulatory proteins. Such pharmacological inhibition allows for identification of key proteins, and for further detailed functional investigation using cell biological, molecular biological, and biochemical methods used for validation of proteomic results. We present a comprehensive approach combining proteomics and cell biology to study vesicular trafficking in plants. Within this approach, we exploit chemical compounds inhibiting particular vesicular trafficking events in plant cells. Treatment of plants with these relatively specific inhibitors results in intracellular accumulation of proteins being transported by vesicles as well as in a change in abundance of regulatory proteins. Such pharmacological inhibition allows for identification of key proteins, and for further detailed functional investigation using cell biological, molecular biological, and biochemical methods used for validation of proteomic results. (en)
Title	Integrative Chemical Proteomics and Cell Biology Methods to Study Endocytosis and Vesicular Trafficking in Arabidopsis Integrative Chemical Proteomics and Cell Biology Methods to Study Endocytosis and Vesicular Trafficking in Arabidopsis (en)
skos:prefLabel	Integrative Chemical Proteomics and Cell Biology Methods to Study Endocytosis and Vesicular Trafficking in Arabidopsis Integrative Chemical Proteomics and Cell Biology Methods to Study Endocytosis and Vesicular Trafficking in Arabidopsis (en)
skos:notation	RIV/61989592:15310/14:33151289!RIV15-MSM-15310___
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(EE2.3.20.0165), P(LO1204)
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Šamaj, Jozef Takáč, Tomáš Šamajová, Olga
http://linked.open.../riv/druhVysledku	C - Kapitola v knize
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Palackého v Olomouci / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	22199
http://linked.open...ai/riv/idVysledku	RIV/61989592:15310/14:33151289
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	LY294002; Wortmannin; Brefeldin A; Inhibitors; 2-D electrophoresis; 2-D LC/MSMS; Plants; Proteomics; Vesicular trafficking; Endocytosis (en)
http://linked.open.../riv/klicoveSlovo	LY294002 Plants Inhibitors 2-D LC/MSMS 2-D electrophoresis Brefeldin A Vesicular trafficking Proteomics Endocytosis Wortmannin
http://linked.open...ontrolniKodProRIV	[B7A6B5610AAC]
http://linked.open...i/riv/mistoVydani	Clifton
http://linked.open...vEdiceCisloSvazku	Methods in Molecular Biology 1209
http://linked.open...i/riv/nazevZdroje	Plant Endosomes: Methods and Protocols
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...v/pocetStranKnihy	286 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Integration of Research Team of the Centre of the Region Haná into International Collaboration Udržitelný rozvoj výzkumu v Centru regionu Haná
http://linked.open...UplatneniVysledku	2014
http://linked.open...iv/tvurceVysledku	Pechan, Tibor Takáč, Tomáš Šamaj, Jozef Šamajová, Olga
number of pages	19 (xsd:int)
http://bibframe.org/vocab/doi	10.1007/978-1-4939-1420-3_20
http://purl.org/ne...btex#hasPublisher	Humana Press
https://schema.org/isbn	978-1-4939-1419-7
http://localhost/t...ganizacniJednotka	15310

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