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Description
  • An enzyme-based amperometric biosensor was developed for the rapid determination of adenine in biological and food samples. Recombinant adenine deaminase (EC 3.5.4.2) from Schizosaccharomyces pombe was co-immobilized on graphite electrode with xanthine oxidase (EC 1.17.3.2), peroxidase (EC 1.11.1.7) and an Os mediator. Optimal working conditions were pH 7.0 and 150 mV vs. Ag/AgCl. The novel method was characterized by a detection limit of 1 m M and a linear measuring range of 0.005-0.1 mM. Different beer and tea samples were analyzed by the newly elaborated adenine biosensor. The results obtained correlate with the adenine content measured by the reference HPLC method.
  • An enzyme-based amperometric biosensor was developed for the rapid determination of adenine in biological and food samples. Recombinant adenine deaminase (EC 3.5.4.2) from Schizosaccharomyces pombe was co-immobilized on graphite electrode with xanthine oxidase (EC 1.17.3.2), peroxidase (EC 1.11.1.7) and an Os mediator. Optimal working conditions were pH 7.0 and 150 mV vs. Ag/AgCl. The novel method was characterized by a detection limit of 1 m M and a linear measuring range of 0.005-0.1 mM. Different beer and tea samples were analyzed by the newly elaborated adenine biosensor. The results obtained correlate with the adenine content measured by the reference HPLC method. (en)
Title
  • Enzyme based amperometric biosensor for adenine determination
  • Enzyme based amperometric biosensor for adenine determination (en)
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  • Enzyme based amperometric biosensor for adenine determination
  • Enzyme based amperometric biosensor for adenine determination (en)
skos:notation
  • RIV/61989592:15310/13:33147808!RIV14-MSM-15310___
http://linked.open...avai/riv/aktivita
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  • P(MEB040906)
http://linked.open...iv/cisloPeriodika
  • 1 - SI
http://linked.open...vai/riv/dodaniDat
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  • 73080
http://linked.open...ai/riv/idVysledku
  • RIV/61989592:15310/13:33147808
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  • determination; amperometric biosensor; adenine deaminase (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV
  • [9DFE89EC4C48]
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  • Electroanalysis
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  • 25
http://linked.open...iv/tvurceVysledku
  • Frébort, Ivo
  • Kiss, Attila
  • Adányi, Nora
  • Boka, Beata
  • Virág, Diana
http://linked.open...ain/vavai/riv/wos
  • 000313807600030
issn
  • 1040-0397
number of pages
http://bibframe.org/vocab/doi
  • 10.1002/elan.201200387
http://localhost/t...ganizacniJednotka
  • 15310
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