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  • Here we present the results of Bacillus subtilis spores breaking using superheating. The spore sample was pumped through the open-ended capillary tube mounted across the heated zone. We investigated the influence of temperature in the range 120-180 degrees C. The heat exposure was controlled by the length of the heated zone, the inner diameter of the capillary and the sample flow rate. We found that spore treatment above 120 degrees C resulted in the release of DNA within 20 s.
  • Here we present the results of Bacillus subtilis spores breaking using superheating. The spore sample was pumped through the open-ended capillary tube mounted across the heated zone. We investigated the influence of temperature in the range 120-180 degrees C. The heat exposure was controlled by the length of the heated zone, the inner diameter of the capillary and the sample flow rate. We found that spore treatment above 120 degrees C resulted in the release of DNA within 20 s. (en)
Title
  • Fast spore breaking by superheating
  • Fast spore breaking by superheating (en)
skos:prefLabel
  • Fast spore breaking by superheating
  • Fast spore breaking by superheating (en)
skos:notation
  • RIV/61989592:15310/13:33147345!RIV14-MSM-15310___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(ED2.1.00/03.0058), P(EE2.3.20.0018)
http://linked.open...iv/cisloPeriodika
  • 9
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
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http://linked.open...dnocenehoVysledku
  • 74730
http://linked.open...ai/riv/idVysledku
  • RIV/61989592:15310/13:33147345
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • disruption; germination; heat; cells; resistance; DNA analysis; anthrax spores; bacillus-subtilis; bacterial-spores; sample preparation method (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV
  • [E2E9627F040E]
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  • Lab on a cip
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http://linked.open...vavai/riv/projekt
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http://linked.open...v/svazekPeriodika
  • 13
http://linked.open...iv/tvurceVysledku
  • Petr, Jan
  • Ševčík, Juraj
  • Neužil, Pavel
  • Přibylka, Adam
  • Almeida, Ana Vanessa
  • Altmeyer, Matthias O
  • Manz, Andreas
http://linked.open...ain/vavai/riv/wos
  • 000316962700005
issn
  • 1473-0197
number of pages
http://bibframe.org/vocab/doi
  • 10.1039/c3lc41305e
http://localhost/t...ganizacniJednotka
  • 15310
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